HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brice, G. T. Articles by Doolan, D. L. Search for Related Content PubMed PubMed Citation Articles by Brice, G. T. Articles by Doolan, D. L.

(Journal of Leukocyte Biology. 2002;72:1164-1171.)
© 2002 by Society for Leukocyte Biology

Optimal induction of antigen-specific CD8+ T cell responses requires bystander cell participation

Gary T. Brice^*, Norma L. Graber^*,, Daniel J. Carucci^* and Denise L. Doolan^*,

^* Malaria Program, Naval Medical Research Center, Silver Spring, Maryland;
Henry M. Jackson Foundation, Rockville, Maryland; and
Department of Molecular Microbiology and Immunology, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland

Correspondence: Dr. Denise L. Doolan, Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Lab 3W16, Silver Spring, MD 20910. E-mail: dooland{at}nmrc.navy.mil

Efficient activation of specific immune responses requires a concerted interaction between T cells and antigen-presenting cells. A requirement for bystander participation of CD4+ T cells for expansion and maintenance of memory CD8+ T cells has been noted in several models, but a role with regard to effector CD8+ T responses has not been well-defined. In this report, the requirement of bystander participation for optimal induction of antigen-specific CD8+ T cell effector function was determined by directly quantitating antigen-specific interferon- (IFN-) CD8+ T cell responses by enzyme-linked immunospot assays, and by indirectly evaluating induction of the chemokine monokine induced by IFN- as a marker for IFN--mediated effector function. Our results demonstrate that bystander cell participation, mediated by CD4+ T cell and natural killer (NK) cells, is required for optimal induction of antigen-specific CD8+ T cell effector responses. Our data further establish a novel role for NK cells in the activation of antigen-specific immune responses.

Key Words: chemokines • cytokines • FACS • T lymphocytes • bystander activation

This article has been cited by other articles:

				C. Brando, L. A. Ware, H. Freyberger, A. Kathcart, A. Barbosa, S. Cayphas, M.-A. Demoitie, P. Mettens, D. G. Heppner, and D. E. Lanar Murine Immune Responses to Liver-Stage Antigen 1 Protein FMP011, a Malaria Vaccine Candidate, Delivered with Adjuvant AS01B or AS02A Infect. Immun., February 1, 2007; 75(2): 838 - 845. [Abstract] [Full Text] [PDF]