HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wirleitner, B. Articles by Fuchs, D. Search for Related Content PubMed PubMed Citation Articles by Wirleitner, B. Articles by Fuchs, D.

(Journal of Leukocyte Biology. 2002;72:1148-1153.)
© 2002 by Society for Leukocyte Biology

Monocyte-derived dendritic cells release neopterin

Barbara Wirleitner, Daniela Reider^*, Susanne Ebner^*, Günther Böck, Bernhard Widner, Matthias Jaeger, Harald Schennach, Nikolaus Romani^* and Dietmar Fuchs^,||

Institutes for
Medical Chemistry and Biochemistry, and for
Experimental Pathology, University of Innsbruck and
^* Department of Dermatology, University of Innsbruck;
Central Institute for Blood Transfusion and Immunology, University Hospital, Innsbruck; and
^|| Ludwig Boltzmann Institute of AIDS-Research, Innsbrugh, Austria

Correspondence: Dr. Dietmar Fuchs, Institute of Medical Chemistry and Biochemistry, University of Innsbruck, Fritz-Pregl-Strasse 3, A-6020 Innsbruck, Austria. E-mail: Dietmar.Fuchs{at}uibk.ac.at

Increased neopterin concentrations in body fluids are found in diseases associated with activated, cell-mediated immunity including infections, autoimmune diseases, and certain malignancies. Monocytes/macrophages are known to secrete large amounts of neopterin upon stimulation with interferon- (IFN-). Ontogenetically, the major part of dendritic cells (DC) belongs to the myeloid lineage. Therefore, we investigated whether cultured monocyte-derived DC can elaborate neopterin. Cells were treated with cytokines in the presence or absence of monocyte-conditioned medium as a maturation stimulus. DC secreted an average 3.5 nmol/l neopterin. In response to IFN-, cells significantly increased their output of neopterin. In distinction to monocytes/macrophages, neopterin production in DC was highly sensitive to IFN- and IFN-ß. Further, lipopolysaccharides (LPS) enhanced neopterin synthesis, whereas tumor necrosis factor , interleukin (IL)-1ß, IL-2, IL-10, and IL-18 were ineffective. Simultaneously, tryptophan degradation by induction of indoleamine (2,3)-dioxygenase (IDO) was tested in stimulated cells. Our results showed that IFN- as well as LPS are inducers of IDO in DC.

Key Words: IFN- • pteridines • tryptophan degradation

This article has been cited by other articles:

				A. A. Byrnes, D. M. Harris, S. F. Atabani, B. P. Sabundayo, S. J. Langan, J. B. Margolick, and C. L. Karp Immune activation and IL-12 production during acute/early HIV infection in the absence and presence of highly active, antiretroviral therapy J. Leukoc. Biol., December 1, 2008; 84(6): 1447 - 1453. [Abstract] [Full Text] [PDF]

				M. S. von Bergwelt-Baildon, A. Popov, T. Saric, J. Chemnitz, S. Classen, M. S. Stoffel, F. Fiore, U. Roth, M. Beyer, S. Debey, et al. CD25 and indoleamine 2,3-dioxygenase are up-regulated by prostaglandin E2 and expressed by tumor-associated dendritic cells in vivo: additional mechanisms of T-cell inhibition Blood, July 1, 2006; 108(1): 228 - 237. [Abstract] [Full Text] [PDF]

				R. Sghiri, J. Feinberg, F. Thabet, K. Dellagi, J. Boukadida, A. Ben Abdelaziz, J. L. Casanova, and M. R. Barbouche Gamma Interferon Is Dispensable for Neopterin Production In Vivo Clin. Vaccine Immunol., December 1, 2005; 12(12): 1437 - 1441. [Abstract] [Full Text] [PDF]

				K. Schroecksnadel, B. Frick, and D. Fuchs Re: Plasma Folate, Vitamin B6, Vitamin B12, Homocysteine, and Risk of Breast Cancer J Natl Cancer Inst, July 16, 2003; 95(14): 1091 - 1091. [Full Text] [PDF]