PeproTech Inc.

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Svensson, L.
Right arrow Articles by Wennerås, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Svensson, L.
Right arrow Articles by Wennerås, C.
(Journal of Leukocyte Biology. 2002;72:810-818.)
© 2002 by Society for Leukocyte Biology

The chemoattractant Trp-Lys-Tyr-Met-Val-D-Met activates eosinophils through the formyl peptide receptor and one of its homologues, formyl peptide receptor-like 1

Lena Svensson*, Claes Dahlgren{dagger} and Christine Wennerås*

* Department of Clinical Bacteriology, Sahlgrenska University Hospital, Göteborg, Sweden; and
{dagger} Department of Rheumatology and Inflammation Research, Göteborg University, Sweden

Correspondence: Christine Wennerås, Department of Clinical Bacteriology, Sahlgrenska University Hospital, Guldhedsgatan 10, S-413 46 Göteborg, Sweden. E-mail: christine.wenneras{at}microbio.gu.se

Whereas prokaryotes use L- and D-isomers of amino acids in their protein synthesis, eukaryotic proteins as a rule incorporate only L-isomers. Hence, D-isomers may constitute danger signals to the innate immune system. A D-methionine-containing peptide, Trp-Lys-Tyr-Met-Val-D-Met-NH2 (WKYMVm), has been shown to be a stronger activator of neutrophils than f-Met-Leu-Phe. The aim of this study was to compare the responsiveness of eosinophils to WKYMVm with that of neutrophils. The peptide was found to induce chemotaxis and respiratory burst in eosinophils. However, it did not mobilize granule constituents, as evidenced by a lack of eosinophil cationic protein, eosinophil peroxidase, and interleukin-5 in the supernatants of stimulated eosinophils. In contrast, WKYMVm caused the release of complement receptor 3 from secretory vesicles in neutrophils. Different members of the formyl peptide receptor family were preferentially engaged by the peptide in the two classes of granulocytes: the formyl peptide receptor itself in eosinophils and formyl peptide receptor-like 1 in neutrophils.

Key Words: degranulation • chemotaxis • respiratory burst • eosinophil cationic protein • eosinophil peroxidase




This article has been cited by other articles:


Home page
 J. Immunol.Home page
T. Devosse, A. Guillabert, N. D'Haene, A. Berton, P. De Nadai, S. Noel, M. Brait, J.-D. Franssen, S. Sozzani, I. Salmon, et al.
Formyl Peptide Receptor-Like 2 Is Expressed and Functional in Plasmacytoid Dendritic Cells, Tissue-Specific Macrophage Subpopulations, and Eosinophils
J. Immunol., April 15, 2009; 182(8): 4974 - 4984.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. M. Brooks, M. E. Bates, R. F. Vrtis, N. N. Jarjour, P. J. Bertics, and J. B. Sedgwick
Urokinase-Type Plasminogen Activator Modulates Airway Eosinophil Adhesion in Asthma
Am. J. Respir. Cell Mol. Biol., October 1, 2006; 35(4): 503 - 511.
[Abstract] [Full Text] [PDF]