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(Journal of Leukocyte Biology. 2002;72:769-779.)
© 2002 by Society for Leukocyte Biology

Human eosinophils express and release IL-13 following CD28-dependent activation

Gaetane Woerly*, Paige Lacy{dagger}, Amena Ben Younes*, Nadine Roger*,{ddagger}, Sylvie Loiseau*, Redwan Moqbel{dagger} and Monique Capron*,{ddagger}

* Centre d’Immunologie et Biologie Parasitaire, Unité INSERM U547 and IFR17, Institut Pasteur, Lille, France;
{dagger} Pulmonary Research Group, Department of Medicine, University of Alberta, Edmonton, Canada; and
{ddagger} Faculté de Pharmacie, Université de Lille 2, France

Correspondence: Dr. Monique Capron, Unité INSERM U547, Institut Pasteur de Lille, 1 rue du Prof. Calmette, BP 245, 59019 Lille Cedex, France. E-mail: monique.capron{at}pasteur-lille.fr

Human eosinophils produce a large number of cytokines, including immunoregulatory cytokines. Given that eosinophils store and release interleukin (IL)-4, a key cytokine in the pathogenesis of allergic inflammation, and that IL-4 and IL-13 share common biological functions, we investigated the possibility that IL-13 may be synthesized by these cells. Using flow cytometry and immunocytochemistry, we show that eosinophils synthesize and store IL-13. Granule localization was demonstrated after subcellular fractionation, and IL-13 immunoreactivity was localized to crystalloid, granule-enriched fractions. Furthermore, electron microscopic analyses specifically localized IL-13 to the dense cores of bicompartmental secondary granules. Upon CD28 ligation, IL-13 was released by eosinophils, whereas a combination of CD28 and immunoglobulin A complexes resulted in decreased IL-13 secretion. Furthermore, eosinophil-derived IL-13 exerts a biological effect, inducing CD23 expression on B cells. By having the capacity to synthesize and release IL-13, eosinophils may participate in the development and maintenance of the T helper cell type 2 response, a prominent feature of allergic diseases.

Key Words: granulocytes • cytokines • flow cytometry • electron microscopy • secretion • inflammation




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