| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Granulocyte Research Laboratory, Department of Hematology, Rigshospitalet, Copenhagen, Denmark; and
Department of Cell Biology, The Netherlands Cancer Institute, Amsterdam
Correspondence: Lene Udby, M.D., Granulocyte Research Laboratory, Department of Hematology L-9322, Rigshospitalet, 9 Blegdamsvej, DK-2100 Copenhagen Ø, Denmark. E-mail: l.udby{at}rh.dk
Cysteine-rich secretory protein 3 (CRISP-3; also known as SGP28) was originally discovered in human neutrophilic granulocytes. We have recently developed a sensitive sandwich enzyme-linked immunosorbent assay for CRISP-3 and demonstrated the presence of CRISP-3 in exocrine secretions. To investigate the subcellular localization and mobilization of CRISP-3 in human neutrophils, we performed subcellular fractionation of resting and activated neutrophils on three-layer Percoll density gradients, release-studies of granule proteins in response to different secretagogues, and double-labeling immunogold electron microscopy. CRISP-3 was found to be localized in a subset of granules with overlapping characteristics of specific and gelatinase granules and mobilized accordingly, thus confirming the hypothesis that peroxidase-negative granules exist as a continuum from specific to gelatinase granules regarding protein content and mobilization. CRISP-3 was found to be a matrix protein, which is stored in granules as glycosylated and as unglycosylated protein. The subcellular distribution of the two forms of CRISP-3 was identical. In addition, CRISP-3 was found as a granule protein in eosinophilic granulocytes. The presence of CRISP-3 in peroxidase-negative granules of neutrophils, in granules of eosinophils, and in exocrine secretions indicates a role in the innate host defense.
Key Words: specific granules • gelatinase granules • SGP28 • immunogold electron microscopy • subcellular fractionation • granulocytes
This article has been cited by other articles:
|
|
 |
|
  G. M. Gibbs, K. Roelants, and M. K. O'Bryan The CAP Superfamily: Cysteine-Rich Secretory Proteins, Antigen 5, and Pathogenesis-Related 1 Proteins--Roles in Reproduction, Cancer, and Immune Defense Endocr. Rev., December 1, 2008; 29(7): 865 - 897. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Jamsai, D. M Bianco, S. J Smith, D. J Merriner, J. D Ly-Huynh, A. Herlihy, B. Niranjan, G. M Gibbs, and M. K O'Bryan Characterization of gametogenetin 1 (GGN1) and its potential role in male fertility through the interaction with the ion channel regulator, cysteine-rich secretory protein 2 (CRISP2) in the sperm tail Reproduction, June 1, 2008; 135(6): 751 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Bjartell, H. Al-Ahmadie, A. M. Serio, J. A. Eastham, S. E. Eggener, S. W. Fine, L. Udby, W. L. Gerald, A. J. Vickers, H. Lilja, et al. Association of Cysteine-Rich Secretory Protein 3 and {beta}-Microseminoprotein with Outcome after Radical Prostatectomy Clin. Cancer Res., July 15, 2007; 13(14): 4130 - 4138. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Busso, N. M. Goldweic, M. Hayashi, M. Kasahara, and P. S. Cuasnicu Evidence for the Involvement of Testicular Protein CRISP2 in Mouse Sperm-Egg Fusion Biol Reprod, April 1, 2007; 76(4): 701 - 708. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Theilgaard-Monch, L. C. Jacobsen, M. J. Nielsen, T. Rasmussen, L. Udby, M. Gharib, P. D. Arkwright, A. F. Gombart, J. Calafat, S. K. Moestrup, et al. Haptoglobin is synthesized during granulocyte differentiation, stored in specific granules, and released by neutrophils in response to activation Blood, July 1, 2006; 108(1): 353 - 361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Nolan, L. Wu, H. J. Bang, S. A. Jelinsky, K. P. Roberts, T. T. Turner, G. S. Kopf, and D. S. Johnston Identification of Rat Cysteine-Rich Secretory Protein 4 (Crisp4) as the Ortholog to Human CRISP1 and Mouse Crisp4 Biol Reprod, May 1, 2006; 74(5): 984 - 991. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Gibbs, M. J. Scanlon, J. Swarbrick, S. Curtis, E. Gallant, A. F. Dulhunty, and M. K. O'Bryan The Cysteine-rich Secretory Protein Domain of Tpx-1 Is Related to Ion Channel Toxins and Regulates Ryanodine Receptor Ca2+ Signaling J. Biol. Chem., February 17, 2006; 281(7): 4156 - 4163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Theilgaard-Monch, L. C. Jacobsen, T. Rasmussen, C. U. Niemann, L. Udby, R. Borup, M. Gharib, P. D. Arkwright, A. F. Gombart, J. Calafat, et al. Highly glycosylated {alpha}1-acid glycoprotein is synthesized in myelocytes, stored in secondary granules, and released by activated neutrophils J. Leukoc. Biol., August 1, 2005; 78(2): 462 - 470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Udby, A. Bjartell, J. Malm, A. Egesten, A. Lundwall, J. B. Cowland, N. Borregaard, and L. Kjeldsen Characterization and Localization of Cysteine-Rich Secretory Protein 3 (CRISP-3) in the Human Male Reproductive Tract J Androl, May 1, 2005; 26(3): 333 - 342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Guo, M. Teng, L. Niu, Q. Liu, Q. Huang, and Q. Hao Crystal Structure of the Cysteine-rich Secretory Protein Stecrisp Reveals That the Cysteine-rich Domain Has a K+ Channel Inhibitor-like Fold J. Biol. Chem., April 1, 2005; 280(13): 12405 - 12412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. U. Niemann, J. B. Cowland, P. Klausen, J. Askaa, J. Calafat, and N. Borregaard Localization of serglycin in human neutrophil granulocytes and their precursors J. Leukoc. Biol., August 1, 2004; 76(2): 406 - 415. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
