Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina
Correspondence: Dr. María Cristina Pistoresi-Palencia, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Ala 1 Pabellón Argentina (5000), Córdoba, Argentina. E-mail: cpistore{at}bioclin.fcq.unc.edu.ar
We examined whether CpG-DNA could be used as adjuvant to induce a T
helper cell type-1 (Th1) immunity in aged BALB/c mice that showed a Th2
polarization. Bordetella pertussis and complete Freunds
adjuvant (CFA) were used as well. Immunization with ovalbumin
(OVA)/CpG-DNA showed that the immunoglobulin G (IgG)2a/IgG1 ratio and
OVA-specific T cell response were similar in young and aged mice.
OVA/CpG-DNA induced the secretion of interferon-
(IFN-
) and
absence of interleukin (IL)-5. Similar results were found in mice
immunized with OVA/CFA. When mice were immunized with OVA/B.
pertussis, we found that the IgG2a/IgG1 ratio and OVA-specific T
cell response were lower in aged mice and elicited IFN-
and IL-5. In
vitro CpG-DNA stimulated antigen-presenting cells to display IL-12 and
up-regulate the expression of major histocompatibility complex class II
and B7-2 on B cells as efficiently in aged as in young mice, but the
up-regulation of B7-1 was stronger in aged mice. The findings
demonstrate that CpG-DNA is able to induce a young-like Th1 specific
immune response in aged mice.
Key Words: vaccines Th2 cells adjuvant immunosenescence aging
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