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(Journal of Leukocyte Biology. 2002;72:373-381.)
© 2002 by Society for Leukocyte Biology

Murine polymorphonuclear neutrophils produce interferon- in response to pulmonary infection with Nocardia asteroides

Department of Medical Microbiology and Immunology, University of California School of Medicine, University of California, Davis

Correspondence: Blaine L. Beaman, Department of Medical Microbiology and Immunology, University of California School of Medicine, University of California, Davis, CA 95616. E-mail: blbeaman{at}ucdavis.edu

Nocardia asteroides causes an acute, necrotizing pneumonia characterized by extensive infiltration of polymorphonuclear neutrophils (PMNs) into the lungs. Although PMNs have historically been classified as end-point cells, recent investigations have indicated that PMNs have the ability to secrete cytokines such as interleukin (IL)-4 and IL-12. This study investigated the ability of PMNs to produce cytokines in a murine model of N. asteroides pulmonary infection. Flow cytometric analysis demonstrated the production of interferon- (IFN-), but not IL-4, by PMNs in response to this infection. IFN- production correlated with peak infiltration of PMNs into the lungs. Cell sorting and enzyme-linked immunosorbent assay were used to confirm cytokine production by cells with nuclear morphology characteristic of PMNs. This is the first report of IFN- production by neutrophils in response to an infection in vivo. These results suggest that PMNs play an important role in directing the host toward a T helper cell type 1 phenotypic response in the lungs.

Key Words: cytokines • granulocytes • flow cytometry • inflammation

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