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(Journal of Leukocyte Biology. 2002;71:941-949.)
© 2002 by Society for Leukocyte Biology

Flt-3 ligand (FL) drives differentiation of rat bone marrow-derived dendritic cells expressing OX62 and/or CD161 (NKR-P1)

Cynthia S. Brissette-Storkus^*,, J. C. Kettel, T. F. Whitham^,, K. M. Giezeman-Smits, L. A. Villa^,||, D. M. Potter^# and William H. Chambers^,||

^* Eye and Ear Institute and Department of Ophthalmology,
Brain Tumor Center of the University of Pittsburgh Cancer Institute, and the Departments of
Molecular Genetics and Biochemistry,
Neurological Surgery,
^|| Pathology, and
^# Biostatistics, University of Pittsburgh School of Medicine, Pennsylvania

Correspondence: Cynthia S. Brissette-Storkus, 919 Eye and Ear Institute, University of Pittsburgh, 203 Lothrop Street, Pittsburgh, PA 15213. E-mail: cbstork{at}pitt.edu

Bone marrow-derived dendritic cells (DC) of the rat have not been as well characterized as those from the mouse. Here, large quantities of bone marrow-derived rat DC were generated when Flt-3 ligand (FL) was used as an adjunct to granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin-4 (IL-4). These cells displayed a typical DC phenotype, expressing MHC class II, CD54, CD80, CD86, and CD11b/c. These DC also uniformly expressed low levels of CD161 and expressed OX62 in a bimodal distribution. Few cells were recovered from cultures grown without FL, and they failed to express OX62 or CD161. The DC generated with FL were more potent antigen-presenting cells in mixed lymphocyte cultures than cells grown without FL, and among FL-derived cells, the OX62⁺ cells were slightly more stimulatory than OX62^- cells. Thus, FL is a useful cytokine for obtaining large quantities of functional rat DC subsets in vitro.

Key Words: IL-4 • GM-CSF • integrin • MHC • MLR

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