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(Journal of Leukocyte Biology. 2002;71:932-940.)
© 2002 by Society for Leukocyte Biology

Rapid static adhesion of human naïve neutrophil to naïve xenoendothelium under physiologic flow is independent of Gal1,3-gal structures

Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia

Correspondence: Futwan Al-Mohanna, Ph.D., Biological and Medical Research, MBC 03, King Faisal Specialist Hospital and Research Centre, P.O. Box 3354, Riyadh 11211, Saudi Arabia. E-mail: futwan{at}kfshrc.edu.sa

Adhesion interactions under flow have long been known to depend on applied wall shear stress. We investigated the ability of human naïve neutrophils to adhere to xenogeneic endothelial cells under static and flow conditions. We demonstrate that human naïve neutrophils bind to xenogeneic endothelial cells under flow conditions. This binding is dependent on the applied stress and is independent of Gal1,3-gal structures, ICAM-1, or its counter ligands LFA-1 and Mac-1. The binding was rapid and is characterized by stationary attachment with no obvious rolling or change in morphology. This binding leads to a transient increase in intracellular-free calcium levels in xenogeneic but not allogeneic-endothelial cells with occasional oscillations that persist long after the initial contact between the two cell types. Previous activation of xenoendothelium by autologous serum or human TNF- augments binding of human naïve neutrophils to the endothelial cells. Our data suggest novel interaction sites between the xenogeneic endothelial cells and human naïve neutrophils.

Key Words: xenotransplantation • calcium • flow stress

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