Journal of Leukocyte Biology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hampton, M. B.
Right arrow Articles by Winterbourn, C. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hampton, M. B.
Right arrow Articles by Winterbourn, C. C.
(Journal of Leukocyte Biology. 2002;71:775-781.)
© 2002 by Society for Leukocyte Biology

Oxidant-mediated phosphatidylserine exposure and macrophage uptake of activated neutrophils: possible impairment in chronic granulomatous disease

Mark B. Hampton*, Margret C. M. Vissers*, Jacqueline I. Keenan{dagger} and Christine C. Winterbourn*

Departments of
* Pathology and
{dagger} Surgery, Christchurch School of Medicine and Health Sciences, Christchurch, New Zealand

Correspondence: Mark Hampton, Ph.D., Christchurch School of Medicine, P.O. Box 4345, Christchurch, New Zealand. E-mail: mark.hampton{at}chmeds.ac.nz

The removal of neutrophils from inflammatory sites is essential for the resolution of inflammation. Surface changes, including phosphatidylserine exposure, label neutrophils for phagocytosis by macrophages. Here, we demonstrate that externalization of phosphatidylserine and uptake by monocyte-derived macrophages occurred in human neutrophils ingesting Staphylococcus aureus. Both processes were dependent on oxidant production from the neutrophil NADPH oxidase. There was no requirement for myeloperoxidase, and H2O2 was identified as the most likely trigger for PS exposure. We hypothesize that clearance of stimulated neutrophils would be delayed in chronic granulomatous disease (CGD) neutrophils, which lack a functional NADPH oxidase. To explore this possibility, heat-killed S. aureus were injected into the peritoneum of CGD and normal mice. Elevated neutrophil numbers were observed in the inflammatory exudate of the CGD animals, consistent with impaired recognition and clearance.

Key Words: phagocyte • inflammation • apoptosis • hydrogen peroxide




This article has been cited by other articles:


Home page
 Infect. Immun.Home page
R. P. Wilkie, M. C. M. Vissers, M. Dragunow, and M. B. Hampton
A Functional NADPH Oxidase Prevents Caspase Involvement in the Clearance of Phagocytic Neutrophils
Infect. Immun., July 1, 2007; 75(7): 3256 - 3263.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
M. C. M. Vissers and R. P. Wilkie
Ascorbate deficiency results in impaired neutrophil apoptosis and clearance and is associated with up-regulation of hypoxia-inducible factor 1{alpha}
J. Leukoc. Biol., May 1, 2007; 81(5): 1236 - 1244.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
B. E. Steinberg and S. Grinstein
Unconventional Roles of the NADPH Oxidase: Signaling, Ion Homeostasis, and Cell Death
Sci. Signal., March 27, 2007; 2007(379): pe11 - pe11.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
T. A. Fuchs, U. Abed, C. Goosmann, R. Hurwitz, I. Schulze, V. Wahn, Y. Weinrauch, V. Brinkmann, and A. Zychlinsky
Novel cell death program leads to neutrophil extracellular traps
J. Cell Biol., January 16, 2007; 176(2): 231 - 241.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. J. Snelgrove, L. Edwards, A. E. Williams, A. J. Rae, and T. Hussell
In the Absence of Reactive Oxygen Species, T Cells Default to a Th1 Phenotype and Mediate Protection against Pulmonary Cryptococcus neoformans Infection
J. Immunol., October 15, 2006; 177(8): 5509 - 5516.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. M. Condliffe, L. M. C. Webb, G. J. Ferguson, K. Davidson, M. Turner, E. Vigorito, M. Manifava, E. R. Chilvers, L. R. Stephens, and P. T. Hawkins
RhoG Regulates the Neutrophil NADPH Oxidase
J. Immunol., May 1, 2006; 176(9): 5314 - 5320.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
F.-C. Cheah, M. B. Hampton, B. A. Darlow, C. C. Winterbourn, and M. C. M. Vissers
Detection of apoptosis by caspase-3 activation in tracheal aspirate neutrophils from premature infants: relationship with NF-{kappa}B activation
J. Leukoc. Biol., March 1, 2005; 77(3): 432 - 437.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
V. E. Kagan, G. G. Borisenko, B. F. Serinkan, Y. Y. Tyurina, V. A. Tyurin, J. Jiang, S. X. Liu, A. A. Shvedova, J. P. Fabisiak, W. Uthaisang, et al.
Appetizing rancidity of apoptotic cells for macrophages: oxidation, externalization, and recognition of phosphatidylserine
Am J Physiol Lung Cell Mol Physiol, July 1, 2003; 285(1): L1 - L17.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
J. R. Brown, D. Goldblatt, J. Buddle, L. Morton, and A. J. Thrasher
Diminished production of anti-inflammatory mediators during neutrophil apoptosis and macrophage phagocytosis in chronic granulomatous disease (CGD)
J. Leukoc. Biol., May 1, 2003; 73(5): 591 - 599.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the Society for Leukocyte Biology.