Journal of Leukocyte Biology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Corinti, S.
Right arrow Articles by Girolomoni, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Corinti, S.
Right arrow Articles by Girolomoni, G.
(Journal of Leukocyte Biology. 2002;71:652-658.)
© 2002 by Society for Leukocyte Biology

Erythrocytes deliver Tat to interferon-{gamma}-treated human dendritic cells for efficient initiation of specific type 1 immune responses in vitro

Silvia Corinti*, Laura Chiarantini{dagger}, Sabrina Dominici{dagger}, Maria Elena Laguardia{dagger}, Mauro Magnani{dagger} and Giampiero Girolomoni*

* Laboratory of Immunology, Istituto Dermopatico dell’Immacolata, IRCCS, Rome, Italy; and
{dagger} Institute of Biochemistry Giorgio Fornaini, University of Urbino, Italy

Correspondence: Dr. Silvia Corinti, Laboratory of Immunology, Istituto Dermopatico dell’Immacolata, IRCCS, Via Monti di Creta 104, 00167 Roma, Italy. E-mail: s.corinti{at}idi.it

Dendritic cells (DC) can represent an important target for vaccine development against viral infections. Here, we studied whether interferon-{gamma} (IFN-{gamma}) could improve the functions of DC and analyzed human red blood cells (RBC) as a delivery system for Tat protein. Monocyte-derived DC were cultured in human serum and matured with monocyte-conditioned medium (MCM) in the presence or not of IFN-{gamma}. Tat was conjugated to RBC (RBC-Tat) through avidin-biotin bridges. Stimulation of DC with IFN-{gamma} increased the release of interleukin (IL)-12 and tumor necrosis factor-{alpha} and inhibited the production of IL-10. Moreover, IFN-{gamma}-treated DC up-regulated the release of CXCL10 (IP-10) markedly and reduced the secretion of CCL17 TARC significantly, attracting preferentially T-helper (Th)1 and Th2 cells, respectively. DC internalized RBC-Tat efficiently. Compared with DC pulsed with soluble Tat, DC incubated with RBC-Tat elicited specific CD4+ and CD8+ T-cell responses at a much lower antigen dose. DC matured in the presence of MCM were more effective than immature DC in inducing T-cell proliferation and IFN-{gamma} release. Finally, immature and mature DC exposed to IFN-{gamma} were better stimulators of allogeneic T cells and induced a higher IFN-{gamma} production from Tat-specific CD4+ and CD8+ T lymphocytes. In conclusion, erythrocytes appear an effective tool for antigen delivery into DC, and IFN-{gamma} could be used advantageously for augmenting the ability of DC to induce type 1 immune responses.

Key Words: T lymphocytes • AIDS • cytokines • chemokines • vaccination






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the Society for Leukocyte Biology.