| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Glaxo-Heritage Asthma Research Laboratory, Pulmonary Research Group, Department of Medicine, University of Alberta, Edmonton, Alberta, Canada; and
Mucosal Inflammation Research Group, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada
Correspondence: Mark Gilchrist, Room 574 HMRC, University of Alberta, Edmonton, Alberta, Canada T6G 2S2. E-mail: Mark.Gilchrist{at}ualberta.ca
Nitric oxide (NO) is a potent mediator synthesized by a variety of cells involved in inflammatory reactions. We investigated the expression of NO synthase (NOS) in rat peritoneal mast cells (PMC). Small amounts of eNOS mRNA were detected basally, whereas neither mRNA for iNOS nor nNOS was detected in unstimulated PMC. Following stimulation by antigen, interferon-
(IFN-), or anti-CD8 antibody, PMC up-regulated iNOS mRNA expression. In situ RT-PCR confirmed that iNOS mRNA originated from PMC. Production of iNOS protein was confirmed in stimulated PMC by immunohistochemistry. Upon stimulation with antigen, IFN-, or anti-CD8, nitrite production was increased significantly (8.4±0.6, 7.6±0.9, and 6.6±0.9 µM/2x105 cells/48 h NO2-, respectively; P<0.01), whereas unstimulated PMC released 2.1 ± 0.3 µM/2 x 105 cells/48 h NO2-. These findings demonstrate that in vivo-derived PMC transcribe and translate mRNA for NOS and produce NO.
Key Words: interferon- • RT-PCR • L-NMMA
This article has been cited by other articles:
|
|
 |
|
  A.-K. Larsson, M. Back, J. Hjoberg, and S.-E. Dahlen Inhibition of Nitric-Oxide Synthase Enhances Antigen-Induced Contractions and Increases Release of Cysteinyl-Leukotrienes in Guinea Pig Lung Parenchyma: Nitric Oxide as a Protective Factor J. Pharmacol. Exp. Ther., October 1, 2005; 315(1): 458 - 465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Swindle, D. D. Metcalfe, and J. W. Coleman Rodent and Human Mast Cells Produce Functionally Significant Intracellular Reactive Oxygen Species but Not Nitric Oxide J. Biol. Chem., November 19, 2004; 279(47): 48751 - 48759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Vannacci, L. Giannini, M. B. Passani, A. Di Felice, S. Pierpaoli, G. Zagli, O. Fantappie, R. Mazzanti, E. Masini, and P. F. Mannaioni The Endocannabinoid 2-Arachidonylglycerol Decreases the Immunological Activation of Guinea Pig Mast Cells: Involvement of Nitric Oxide and Eicosanoids J. Pharmacol. Exp. Ther., October 1, 2004; 311(1): 256 - 264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gilchrist, S. D. McCauley, and A. D. Befus Expression, localization, and regulation of NOS in human mast cell lines: effects on leukotriene production Blood, July 15, 2004; 104(2): 462 - 469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. L. M. Ricciardolo, P. J. Sterk, B. Gaston, and G. Folkerts Nitric Oxide in Health and Disease of the Respiratory System Physiol Rev, July 1, 2004; 84(3): 731 - 765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gilchrist, C. Hesslinger, and A. D. Befus Tetrahydrobiopterin, a Critical Factor in the Production and Role of Nitric Oxide in Mast Cells J. Biol. Chem., December 12, 2003; 278(50): 50607 - 50614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Marcet-Palacios, K. Graham, C. Cass, A. D. Befus, I. Mayers, and M. W. Radomski Nitric Oxide and Cyclic GMP Increase the Expression of Matrix Metalloproteinase-9 in Vascular Smooth Muscle J. Pharmacol. Exp. Ther., October 1, 2003; 307(1): 429 - 436. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
