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(Journal of Leukocyte Biology. 2002;71:582-587.)
© 2002 by Society for Leukocyte Biology

Rat monocyte-derived dendritic cells function and migrate in the same way as isolated tissue dendritic cells

C. D. Richters^*, I. Mayen^*, C. E. G. Havenith, R. H. J. Beelen^* and E. W. A. Kamperdijk^*

^* Department of Molecular Cell Biology, Faculty of Medicine, VUMC, Amsterdam; and
Division of Immunological and Infectious diseases, TNO Prevention and Health, Leiden, The Netherlands

Correspondence: C. D. Richters, Department of Molecular Cell Biology, Faculty of Medicine, VUMC, Van der Boechorststraat 7, 1081 BT Amsterdam. E-mail: CD.Richters.Cell{at}med.vu.nl

Dendritic cells (DC) are the most potent antigen-presenting cells and are therefore useful to induce immune responses against tumor cells in patients. DC can be generated in vitro from monocytes using GM-CSF and IL-4, the so-called monocyte-derived DC (MoDC). To achieve antitumor responses, MoDC must be able to migrate to the draining lymph nodes after injection to induce cytotoxic T cells. Therefore, we studied migration of MoDC in a rat model. Functional rat MoDC were generated from PVG-RT7B rats and injected subcutaneously into PVG rats. These rat strains differ only at one epitope of the leukocyte-common antigen, which can be recognized by the antibody His 41. The advantage is that migrated cells can be detected in the draining lymph nodes by staining sections with His 41+; thus, migration is not influenced by labeling procedures. Rat MoDC migrated to the T-cell areas of the draining lymph nodes, just as isolated Langerhans cells or spleen DC do. In contrast, monocytes also migrated to the B-cell areas and the medulla.

Key Words: antigen presentation • tumor cells • GM-CSF • Langerhans cells

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