| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Microbiology, National Public Health Institute, Helsinki, Finland
Correspondence: Anne Lehtonen, Laboratory of Viral and Molecular Immunology, Department of Microbiology, National Public Health Institute, Mannerheimintie 166, FIN-00300 Helsinki, Finland. E-mail: Anne.Lehtonen{at}ktl.fi
GM-CSF signals through JAK2 and STAT5 and stimulates the expression of STAT5 target genes, such as pim-1 and CIS. Analyzed by EMSA, GM-CSF stimulation led to much stronger STAT5 DNA-binding to pim-1 or CIS GAS elements in primary human monocytes compared with mature macrophages. Similarly, GM-CSF-induced expression of pim-1 and CIS mRNAs was much stronger in monocytes. These differencies were not a result of downregulation of the GM-CSF receptor system or STAT5 expression, because monocytes and macrophages readily expressed GM-CSF receptor, JAK2, STAT5A, and STAT5B mRNAs and proteins. Monocytes expressed significant amounts of truncated STAT5 forms that took part in STAT5-DNA complex formation in GM-CSF-stimulated monocytes. This resulted in faster moving STAT5 complexes compared with macrophages in EMSA. Our results demonstrate that STAT5 isoform expression, GM-CSF-induced STAT5 activation, and STAT5 target-gene expression are altered significantly during monocyte/macrophage differentiation.
Key Words: signal transduction • cytokines • cellular differentiation
This article has been cited by other articles:
|
|
 |
|
  P. A. Cohen, G. K. Koski, B. J. Czerniecki, K. D. Bunting, X.-Y. Fu, Z. Wang, W.-J. Zhang, C. S. Carter, M. Awad, C. A. Distel, et al. STAT3- and STAT5-dependent pathways competitively regulate the pan-differentiation of CD34pos cells into tumor-competent dendritic cells Blood, September 1, 2008; 112(5): 1832 - 1843. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Tanimoto, Y. Murata, K.-Y. Wang, M. Tsutsui, K. Kohno, and Y. Sasaguri Monocyte Chemoattractant Protein-1 Expression Is Enhanced by Granulocyte-Macrophage Colony-stimulating Factor via Jak2-Stat5 Signaling and Inhibited by Atorvastatin in Human Monocytic U937 Cells J. Biol. Chem., February 22, 2008; 283(8): 4643 - 4651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Crotti, M. Lusic, R. Lupo, P. M. J. Lievens, E. Liboi, G. D. Chiara, M. Tinelli, A. Lazzarin, B. K. Patterson, M. Giacca, et al. Naturally occurring C-terminally truncated STAT5 is a negative regulator of HIV-1 expression Blood, June 15, 2007; 109(12): 5380 - 5389. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X Han, N Benight, B Osuntokun, K Loesch, S J Frank, and L A Denson Tumour necrosis factor {alpha} blockade induces an anti-inflammatory growth hormone signalling pathway in experimental colitis Gut, January 1, 2007; 56(1): 73 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Han, B. Osuntokun, N. Benight, K. Loesch, S. J. Frank, and L. A. Denson Signal Transducer and Activator of Transcription 5b Promotes Mucosal Tolerance in Pediatric Crohn's Disease and Murine Colitis Am. J. Pathol., December 1, 2006; 169(6): 1999 - 2013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Lehtonen, V. Veckman, T. Nikula, R. Lahesmaa, L. Kinnunen, S. Matikainen, and I. Julkunen Differential Expression of IFN Regulatory Factor 4 Gene in Human Monocyte-Derived Dendritic Cells and Macrophages J. Immunol., November 15, 2005; 175(10): 6570 - 6579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Litherland, K. M. Grebe, N. S. Belkin, E. Paek, J. Elf, M. Atkinson, L. Morel, M. J. Clare-Salzler, and M. McDuffie Nonobese Diabetic Mouse Congenic Analysis Reveals Chromosome 11 Locus Contributing to Diabetes Susceptibility, Macrophage STAT5 Dysfunction, and Granulocyte-Macrophage Colony-Stimulating Factor Overproduction J. Immunol., October 1, 2005; 175(7): 4561 - 4565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Larrivee, I. Pollet, and A. Karsan Activation of Vascular Endothelial Growth Factor Receptor-2 in Bone Marrow Leads to Accumulation of Myeloid Cells: Role of Granulocyte-Macrophage Colony-Stimulating Factor J. Immunol., September 1, 2005; 175(5): 3015 - 3024. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Warby, S. M. Crowe, and A. Jaworowski Human Immunodeficiency Virus Type 1 Infection Inhibits Granulocyte-Macrophage Colony-Stimulating Factor-Induced Activation of STAT5A in Human Monocyte-Derived Macrophages J. Virol., December 1, 2003; 77(23): 12630 - 12638. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
