Vancouver

This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Epelman, S.
Right arrow Articles by Mody, C. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Epelman, S.
Right arrow Articles by Mody, C. H.
(Journal of Leukocyte Biology. 2002;71:458-468.)
© 2002 by Society for Leukocyte Biology

Distinct fates of monocytes and T cells directly activated by Pseudomonas aeruginosa exoenzyme S

Slava Epelman*, Graham G. Neely2, Ling Ling Ma2, Mark Gjomarkaj3, Elisabetta Pace3, Mario Melis3, Donald E. Woods* and Christopher H. Mody*

Departments of
* Microbiology and Infectious Diseases,
{dagger} Medical Sciences, and
§ Internal Medicine, University of Calgary, Alberta, Canada; and
{ddagger} Istituto di Fisiopatologia Respiratoria, CNR, Palermo, Italy

Correspondence: Christopher H. Mody, M.D., FRCPC, Rm. 273, Heritage Medical Research Building, 3330 Hospital Dr., N.W., University of Calgary, Calgary, Alberta, Canada, T2N-4N1. E-mail: cmody{at}ucalgary.ca

Gram-negative infections can cause overwhelming inflammatory responses. Although factors other than LPS are clearly involved, these factors and their mechanisms of action have been poorly defined. During studies of LPS-independent inflammatory responses of the gram-negative pathogen Pseudomonas aeruginosa, an important virulence factor (exoenzyme S) was shown to be a potent mitogen for T cells. The current work demonstrates that exoenzyme S selectively induced transcription and secretion of biologically active cytokines and chemokines (chemotactic for neutrophils and T cells) from monocytes. Exoenzyme S stimulated highly purified monocytes independent of T cells. In addition, exoenzyme S stimulated T cells directly; neither T-cell activation (CD69) nor apoptosis (hypodiploidy) required the presence of monocytes. However, T-cell activation was enhanced via a noncontact-dependent mechanism as a result of the secretion of TNF-{alpha} and IL-6. This study identifies a unique property of a gram-negative-derived microbial product capable of activating multiple cell types and suggests a mechanism by which exoenzyme S contributes to the immunopathogenesis of cystic fibrosis and sepsis in patients infected with P. aeruginosa.

Key Words: gram negative • inflammation • apoptosis • mitogen




This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Epelman, B. Berenger, D. Stack, G. G. Neely, L. L. Ma, and C. H. Mody
Microbial Products Activate Monocytic Cells through Detergent-Resistant Membrane Microdomains
Am. J. Respir. Cell Mol. Biol., December 1, 2008; 39(6): 657 - 665.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
H. Abusriwil and R. A. Stockley
The Interaction of Host and Pathogen Factors in Chronic Obstructive Pulmonary Disease Exacerbations and Their Role in Tissue Damage
Proceedings of the ATS, December 1, 2007; 4(8): 611 - 617.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Jia, Y. Wang, L. Zhou, and S. Jin
Expression of Pseudomonas aeruginosa Toxin ExoS Effectively Induces Apoptosis in Host Cells
Infect. Immun., December 1, 2006; 74(12): 6557 - 6570.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Epelman, D. Stack, C. Bell, E. Wong, G. G. Neely, S. Krutzik, K. Miyake, P. Kubes, L. D. Zbytnuik, L. L. Ma, et al.
Different Domains of Pseudomonas aeruginosa Exoenzyme S Activate Distinct TLRs
J. Immunol., August 1, 2004; 173(3): 2031 - 2040.
[Abstract] [Full Text] [PDF]