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(Journal of Leukocyte Biology. 2002;71:195-204.)
© 2002 by Society for Leukocyte Biology

Myosin-V colocalizes with MHC class II in blood mononuclear cells and is up-regulated by T-lymphocyte activation

João C. S. Bizario*,{dagger}, Fabíola A. Castro§,||, Josane F. Sousa*, Rafael N. Fernandes{dagger}, Alexandre D. Damião*, Márika K. Oliveira*, Patrícia V. B. Palma||, Roy E. Larson*, Júlio C. Voltarelli{ddagger},|| and Enilza M. Espreafico*

* Departments of Cellular and Molecular Biology and Pathogenic Bioagents, and
{ddagger} Clinical Medicine of the Faculty of Medicine of Ribeirão Preto, and
§ Faculty of Pharmaceutical Sciences of Ribeirão Preto of the University of São Paulo;
{dagger} Medical School of the Ribeirão Preto University (UNAERP), Brazil; and
|| Blood Center Foundation of Ribeirão Preto, SP, Brazil

Correspondence: Enilza M. Espreafico, Av. Bandeirantes, 3900, Ribeirão Preto, SP, 14049-900, Brazil. E-mail: emesprea{at}fmrp.usp.br

Myosin-V is involved in organelle and vesicle trafficking in Saccharomyces cerevisiae and in other eukaryotic cells from yeast to human. In the present study, we determined by FACS that the major subpopulations of the peripheral blood mononuclear cells express myosin-V with similar fluorescence intensity. Confocal microscopy showed intense labeling for myosin-V at the centrosomal region and a punctate staining throughout the cytoplasm, frequently associated with the central microtubule arrays and the actin-rich cortex. Some degree of overlap with an endolysosomal marker and dynein light-chain 8 k was found at the cell center. Striking colocalization was observed with the major histocompatibility complex (MHC) class II molecules near the cell surface. Treatment with phytohemagglutinin, which induces T-lymphocyte activation, associated with MHC class II expression, increased the levels of myosin-V protein and mRNA for the three members of class V myosins. These data suggest that class V myosins might be involved in relevant functions in the immune response.

Key Words: Griscelli syndrome • centrosome • natural killer cells • Rab27a • dilute gene • endosomes




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