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(Journal of Leukocyte Biology. 2002;71:184-194.)
© 2002 by Society for Leukocyte Biology

NK-like CD8⁺ cells in immunologically naïve neonatal calves that respond to dendritic cells infected with Mycobacterium bovis BCG

Institute for Animal Health, Compton, Newbury, Berkshire, United Kingdom

Correspondence: Dr. J. C. Hope, Institute for Animal Health, Compton, Newbury, Berkshire, RG20 7NN, United Kingdom. E-mail: Jayne.Hope{at}BBSRC.ac.uk

Pre-exposure to environmental mycobacteria and induction of an inappropriately biased immune response may be major factors affecting the efficacy of BCG; vaccination of neonates that have not been exposed to environmental mycobacteria may induce more effective immunity. Responses of neonatal calves to mycobacterial antigens using dendritic cells (DC) as antigen-presenting cells were investigated. In nonvaccinated, immunologically naive calves as young as 1 day old, a population of CD8⁺ cells proliferated and produced IFN- in response to BCG-infected DC. CD3^- CD8⁺ NK-like and CD3⁺ CD8⁺ T cells were evident within the responding CD8⁺ population. The response was not MHC-restricted. The NK-like CD3^- cells were the major population producing IFN-. The presence of mycobacteria-reactive, IFN--secreting CD8⁺ NK cells in neonatal calves may have important consequences for the induction of a Th1-biased immune response.

Key Words: mycobacteria • IFN- • NKT cells • antigen-presenting cells

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