HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hokari, R. Articles by Ishii, H. Search for Related Content PubMed PubMed Citation Articles by Hokari, R. Articles by Ishii, H.

(Journal of Leukocyte Biology. 2001;70:896-902.)
© 2001 by Society for Leukocyte Biology

Intercellular cell adhesion molecule-1 regulates lymphocyte movement into intestinal microlymphatics of rat Peyer’s patches

Ryota Hokari^*, Soichiro Miura^*, Hiroshi Nagata, Hitoshi Fujimori, Seiichiro Koseki, Shingo Kato^*, Iwao Kurose, Eiichi Sekizuka, D. Neil Granger and Hiromasa Ishii

^* Second Department of Internal Medicine, National Defense Medical College, Saitama, Japan;
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan;
Department of Internal Medicine, National Saitama Hospital, Saitama, Japan; and
Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport

Correspondence: Soichiro Miura, M.D., Professor, Second Department of Internal Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan. E-mail: miura{at}me.ndmc.ac.jp

The objective of this study was to determine whether specific adhesion molecules modulate lymphocyte movement from Peyer’s patches into intestinal microlymphatics. The fluorochrome acridine orange was injected via a micropipette into Peyer’s patches to fill lymphatics. The flux of labeled lymphocytes into intestinal microlymphatics was monitored with intravital fluorescence microscopy. The lymphatic microvessels in the perifollicular area of Peyer’s patches were filled with lymphocytes, most of which remained within the lymphatics. Some lymphocytes became detached and were drained into intestinal lymph. Administration of antibodies directed against ICAM-1 significantly increased lymphocyte flux into interfollicular lymphatics. The immunohistochemical study showed intense ICAM-1 expression on the lymphocytes densely packed in the lymphatics surrounding follicles in Peyer’s patches. A large number of lymphocytes are normally sequestered in the lymphatic network of Peyer’s patches. This sequestration of lymphocytes is largely mediated by ICAM-1-dependent cell-cell interactions.

Key Words: CD18 • acridine orange • collecting lymphatics • mesenteric lymphadenectomy

This article has been cited by other articles:

				H. Kobayashi, S. Miura, H. Nagata, Y. Tsuzuki, R. Hokari, T. Ogino, C. Watanabe, T. Azuma, and H. Ishii In situ demonstration of dendritic cell migration from rat intestine to mesenteric lymph nodes: relationships to maturation and role of chemokines J. Leukoc. Biol., March 1, 2004; 75(3): 434 - 442. [Abstract] [Full Text] [PDF]