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(Journal of Leukocyte Biology. 2001;70:881-886.)
© 2001 by Society for Leukocyte Biology

Senescence in innate immune responses: reduced neutrophil phagocytic capacity and CD16 expression in elderly humans

S. K. Butcher^*, H. Chahal^*, L. Nayak, A. Sinclair, N. V. Henriquez^*, E. Sapey, D. O’Mahony and J. M. Lord^*

^* MRC Centre for Immune Regulation, Birmingham University Medical School, Birmingham, United Kingdom; and Departments of
Geriatric Medicine and
Medicine, University Hospital, Birmingham, United Kingdom

Correspondence: Dr. J. M. Lord, MRC Centre for Immune Regulation, Birmingham University Medical School, Birmingham B15 2TT, UK. E-mail: J.M.Lord{at}bham.ac.uk

Elderly humans are more susceptible to bacterial infections because of declining immune status. We have investigated the effect of aging on neutrophil bactericidal responses, comparing neutrophil function in healthy, young (23–35 years) and elderly (>65 years) volunteers. Superoxide generation in response to fMLP was slightly increased in neutrophils from elderly donors, and serum from the elderly was able to opsonize E. coli efficiently. In contrast, phagocytic index was significantly lower in neutrophils from the elderly, compared with young donors (P<0.005). CD11a and CD11b expression was not affected by age, but CD16 was significantly reduced in neutrophils from elderly donors (P<0.0001). CD16 expression and phagocytic index were measured in the same neutrophils using FITC-labeled E. coli, PE-conjugated anti-CD16 antibody, and CD16 expression correlated with phagocytic index (r=0.83; P<0.05). In elderly patients with bacterial infection, CD16 expression remained low. We propose that reduced neutrophil CD16 expression and phagocytosis contribute to human immunesenescence.

Key Words: immunesenescence • aging • neutrophil-function

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