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(Journal of Leukocyte Biology. 2001;70:745-748.)
© 2001 by Society for Leukocyte Biology

Treatment of multiple sclerosis patients with interferon-ß primes monocyte-derived macrophages for apoptotic cell death

Johan Van Weyenbergh*, Juana Wietzerbin*, Dany Rouillard{dagger}, Manoel Barral-Netto{ddagger} and Roland Liblau§

* U365 INSERM,
{dagger} Section de Recherche, Institut Curie
§ U546 INSERM, Hôpital La Pitié-Salpêtrière, Paris, France
{ddagger} Gonçalo Moniz Research Center, Oswaldo Cruz Foundation, Salvador-Bahia, Brazil

Although interferon (IFN)-ß has shown a significant clinical benefit in multiple sclerosis (MS), its mechanism of action remains unclear. We found that IFN-ß treatment of patients with MS resulted in a significant increase in apoptotic cell death (measured by annexin V staining and nuclear fragmentation) of monocyte-derived macrophages, as compared with cells derived from patients before treatment. Stimulation of the cells with IFN-ß in vitro resulted in an even further increase of annexin V binding, as well as increased Fas (CD 95, APO-1) expression. However, no increased Fas expression, apoptotic monocytes, or monocytopenia were observed upon in vivo treatment. This indicates that IFN-ß does not deliver a death signal to monocytes but rather primes for subsequent macrophage apoptosis upon activation or differentiation.

Key Words: in vivo • CD 14 • CD 64 • CD 95 (Fas)




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