HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Van Weyenbergh, J. Articles by Liblau, R. Search for Related Content PubMed PubMed Citation Articles by Van Weyenbergh, J. Articles by Liblau, R.

(Journal of Leukocyte Biology. 2001;70:745-748.)
© 2001 by Society for Leukocyte Biology

Treatment of multiple sclerosis patients with interferon-ß primes monocyte-derived macrophages for apoptotic cell death

Johan Van Weyenbergh^*, Juana Wietzerbin^*, Dany Rouillard, Manoel Barral-Netto and Roland Liblau

^* U365 INSERM,
Section de Recherche, Institut Curie
U546 INSERM, Hôpital La Pitié-Salpêtrière, Paris, France
Gonçalo Moniz Research Center, Oswaldo Cruz Foundation, Salvador-Bahia, Brazil

Although interferon (IFN)-ß has shown a significant clinical benefit in multiple sclerosis (MS), its mechanism of action remains unclear. We found that IFN-ß treatment of patients with MS resulted in a significant increase in apoptotic cell death (measured by annexin V staining and nuclear fragmentation) of monocyte-derived macrophages, as compared with cells derived from patients before treatment. Stimulation of the cells with IFN-ß in vitro resulted in an even further increase of annexin V binding, as well as increased Fas (CD 95, APO-1) expression. However, no increased Fas expression, apoptotic monocytes, or monocytopenia were observed upon in vivo treatment. This indicates that IFN-ß does not deliver a death signal to monocytes but rather primes for subsequent macrophage apoptosis upon activation or differentiation.

Key Words: in vivo • CD 14 • CD 64 • CD 95 (Fas)

This article has been cited by other articles:

				G. H. Fernald, S. Knott, A. Pachner, S. J. Caillier, K. Narayan, J. R. Oksenberg, P. Mousavi, and S. E. Baranzini Genome-Wide Network Analysis Reveals the Global Properties of IFN-beta Immediate Transcriptional Effects in Humans J. Immunol., April 15, 2007; 178(8): 5076 - 5085. [Abstract] [Full Text] [PDF]