B activation but not for phagocytosis
Immunology Department, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City
Correspondence: Dr. Carlos Rosales, Department of Immunology, Instituto de Investigaciones Biomédicas—UNAM, Apto. Postal 70228, Cd. Universitaria, México D.F.-04510, Mexico. E-mail: carosal{at}servidor.unam.mx
The molecular events that transduce signals from Fc receptors to
the various cellular responses are still poorly defined. We have
investigated the role of phosphatidylinositol 3-kinase (PI 3-K) and
extracellular signal-regulated kinase (ERK) in gene activation and
phagocytosis in monocytes. In the THP-1 monocytic cell line,
cross-linking of Fc receptors by immune complexes results in activation
of the transcription factor NF-
B, via activation of ERK. Activation
of both ERK and NF-B was blocked by wortmannin and LY294002,
specific inhibitors of PI 3-K. Wortmannin also inhibited the Fc
receptor-mediated increase in the cytosolic calcium concentration, but
it did not block immunoglobulin G (IgG)-mediated phagocytosis. In
addition, the ERK inhibitor PD98059 did not block phagocytosis of
IgG-coated erythrocytes. Both the increase in the cytosolic calcium
concentration and phagocytosis depend on an active actin cytoskeleton,
as indicated by the total lack of both responses after treatment with
cytochalasin B. In contrast, cytochalasin B did not affect Fc
receptor-mediated activation of NF-B. These results identify PI 3-K
and ERK as important signaling molecules in the Fc receptor signal
transduction pathway of monocytes, which leads to the nucleus for gene
activation. These results also suggest that, in contrast to other cell
types, unstimulated monocytes do not require PI 3-K and ERK for
phagocytosis.
Key Words: monocytes • signal transduction • Fc receptors
This article has been cited by other articles:
 |
|
 |
|
  E. Garcia-Garcia, G. Nieto-Castaneda, M. Ruiz-Saldana, N. Mora, and C. Rosales Fc{gamma}RIIA and Fc{gamma}RIIIB Mediate Nuclear Factor Activation through Separate Signaling Pathways in Human Neutrophils J. Immunol., April 15, 2009; 182(8): 4547 - 4556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Lee, W. M. Nauseef, A. Moeenrezakhanlou, L. M. Sly, S. Noubir, K. G. Leidal, J. M. Schlomann, G. Krystal, and N. E. Reiner Monocyte p110{alpha} phosphatidylinositol 3-kinase regulates phagocytosis, the phagocyte oxidase, and cytokine production J. Leukoc. Biol., June 1, 2007; 81(6): 1548 - 1561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Sawatzky, D. A. Willoughby, P. R. Colville-Nash, and A. G. Rossi The Involvement of the Apoptosis-Modulating Proteins ERK 1/2, Bcl-xL and Bax in the Resolution of Acute Inflammation in Vivo Am. J. Pathol., January 1, 2006; 168(1): 33 - 41. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Harfi, F. Corazza, S. D'Hondt, and E. Sariban Differential Calcium Regulation of Proinflammatory Activities in Human Neutrophils Exposed to the Neuropeptide Pituitary Adenylate Cyclase-Activating Protein J. Immunol., September 15, 2005; 175(6): 4091 - 4102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ortiz-Stern and C. Rosales Fc{gamma}RIIIB stimulation promotes {beta}1 integrin activation in human neutrophils J. Leukoc. Biol., May 1, 2005; 77(5): 787 - 799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Poulaki, A. M. Joussen, N. Mitsiades, C. S. Mitsiades, E. F. Iliaki, and A. P. Adamis Insulin-Like Growth Factor-I Plays a Pathogenetic Role in Diabetic Retinopathy Am. J. Pathol., August 1, 2004; 165(2): 457 - 469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Song, S. Tanaka, D. Cox, and S. C. Lee Fc{gamma} receptor signaling in primary human microglia: differential roles of PI-3K and Ras/ERK MAPK pathways in phagocytosis and chemokine induction J. Leukoc. Biol., June 1, 2004; 75(6): 1147 - 1155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Shiratsuchi and M. D. Basson Extracellular pressure stimulates macrophage phagocytosis by inhibiting a pathway involving FAK and ERK Am J Physiol Cell Physiol, June 1, 2004; 286(6): C1358 - C1366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Garcia-Garcia and C. Rosales Signal transduction during Fc receptor-mediated phagocytosis J. Leukoc. Biol., December 1, 2002; 72(6): 1092 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Marrache, F. Gobeil Jr., S. G. Bernier, J. Stankova, M. Rola-Pleszczynski, S. Choufani, G. Bkaily, A. Bourdeau, M. G. Sirois, A. Vazquez-Tello, et al. Proinflammatory Gene Induction by Platelet-Activating Factor Mediated Via Its Cognate Nuclear Receptor J. Immunol., December 1, 2002; 169(11): 6474 - 6481. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Song, S. Shapiro, D. L. Goldman, A. Casadevall, M. Scharff, and S. C. Lee Fc{gamma} Receptor I- and III-Mediated Macrophage Inflammatory Protein 1{alpha} Induction in Primary Human and Murine Microglia Infect. Immun., September 1, 2002; 70(9): 5177 - 5184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Garcia-Garcia, R. Rosales, and C. Rosales Phosphatidylinositol 3-kinase and extracellular signal-regulated kinase are recruited for Fc receptor-mediated phagocytosis during monocyte-to-macrophage differentiation J. Leukoc. Biol., July 1, 2002; 72(1): 107 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Gobeil Jr, I. Dumont, A. M. Marrache, A. Vazquez-Tello, S. G. Bernier, D. Abran, X. Hou, M. H. Beauchamp, C. Quiniou, A. Bouayad, et al. Regulation of eNOS Expression in Brain Endothelial Cells by Perinuclear EP3 Receptors Circ. Res., April 5, 2002; 90(6): 682 - 689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Gobeil Jr, I. Dumont, A. M. Marrache, A. Vazquez-Tello, S. G. Bernier, D. Abran, X. Hou, M. H. Beauchamp, C. Quiniou, A. Bouayad, et al. Regulation of eNOS Expression in Brain Endothelial Cells by Perinuclear EP3 Receptors Circ. Res., April 5, 2002; 90(6): 682 - 689. [Abstract] [Full Text] [PDF]
|
|
