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(Journal of Leukocyte Biology. 2001;70:297-305.)
© 2001 by Society for Leukocyte Biology

Early effects of insulin-like growth factor-1 in activated human T lymphocytes

Mariana G. Brocardo*, Roxana Schillaci{dagger}, Adriana Galeano{ddagger}, Martín Radrizzani*, Verónica White{dagger}, Anatilde González Guerrico*, Tomás A. Santa-Coloma* and Alicia Roldán{dagger}

* Instituto de Investigaciones Bioquímicas Fundación Campomar (IIB-UBA and IIBBA CONICET),
{dagger} Instituto de Biología y Medicina Experimental CONICET, and
{ddagger} Laboratorio de Patología Sanatorio Mater Dei, Buenos Aires, Argentina

Correspondence: Alicia Roldán, Instituto de Biología y Medicina Experimental, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina. E-mail: aroldan{at}dna.uba.ar

This study evaluates the effects of insulin-like growth factor (IGF)-1 receptor (IGF-1R) down-regulation in stimulated T lymphocytes by investigating the expression of early activation proteins CD69, CD25, and interleukin (IL)-2. We found that IGF-1 does not modify CD69 expression but increases transcription and protein synthesis of CD25 and IL-2. The lowest level of IGF-1R detected after 15 min of activation suggested that the effects of IGF-1 occur at the initiation of cell activation. The activation of IGF-1R was confirmed by IGF-1R phosphorylation and increased phosphorylation of microtubule-associated protein kinase. We also detected the alternative IGF-1 transcripts Ea, with paracrine/autocrine regulation, and Eb, with endocrine regulation, in Jurkat cells and in quiescent T lymphocytes, and we detected IGF-1 protein in the culture medium after stimulation. These data suggest that the proliferative effects of IGF-1 on T lymphocytes include both autocrine/paracrine and endocrine processes.

Key Words: CD25 • IL-2 • IGF-1 receptor • IGF-1 mRNA • MAPK




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