* Laboratory of Immunology, Department of Clinical Biochemistry, Faculty of Chemical Sciences, National University of Córdoba, and
Laboratory of Immunogenetics, Faculty of Medicine, and
Department of Biological Chemistry, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina
Correspondence: Dr. Adriana Gruppi, Departamento de Bioqu
ica Cli
ica, Facultad de Ciencias Quicas, Universidad Nacional de Córdoba 5000, Cordoba CC61, Argentina. E-mail: agruppi{at}bioclin.fcq.unc.edu.ar
Galectin-1 (GAL-1), a highly conserved ß-galactoside-binding protein,
has shown immunomodulatory properties. In this study, we investigated
the regulation of GAL-1 expression within the B-cell compartment using
Trypanosoma cruzi infection as a natural model of in vivo
B-cell activation. GAL-1 was found to be expressed on activated B cells
from T. cruzi-infected mice, mainly localized at the
cytosolic compartment. Expression of this protein was found to be
modulated according to the activation state of the cells, revealing a
significant increase in stimulated B cells that received signals via
cross-linking of the B-cell receptor and CD40. It was found that GAL-1
was secreted by B cells to the extracellular milieu upon activation.
Finally, purified GAL-1 produced by activated B cells induced apoptosis
of T cells but not B cells and also influenced interferon-
cytokine
production. Hence, the present study describes a potential mechanism by
which B cells can regulate T-cell function and survival.
Key Words: B lymphocytes • galectin-1 • Trypanosoma cruzi
This article has been cited by other articles:
 |
|
 |
|
  K. Loser, A. Sturm, M. Voskort, V. Kupas, S. Balkow, M. Auriemma, C. Sternemann, A. U. Dignass, T. A. Luger, and S. Beissert Galectin-2 Suppresses Contact Allergy by Inducing Apoptosis in Activated CD8+ T Cells J. Immunol., May 1, 2009; 182(9): 5419 - 5429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-M. Tsai, Y.-K. Chiu, T.-L. Hsu, I-Y. Lin, S.-L. Hsieh, and K.-I Lin Galectin-1 Promotes Immunoglobulin Production during Plasma Cell Differentiation J. Immunol., October 1, 2008; 181(7): 4570 - 4579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. I. Garin, C.-C. Chu, D. Golshayan, E. Cernuda-Morollon, R. Wait, and R. I. Lechler Galectin-1: a key effector of regulation mediated by CD4+CD25+ T cells Blood, March 1, 2007; 109(5): 2058 - 2065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Yu, R. Siegel, and R. G. Roeder Interaction of the B Cell-specific Transcriptional Coactivator OCA-B and Galectin-1 and a Possible Role in Regulating BCR-mediated B Cell Proliferation J. Biol. Chem., June 2, 2006; 281(22): 15505 - 15516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Xu, C.-T. Shu, E. Purdom, D. Dang, D. Ilsley, Y. Guo, J. Weber, S. P. Holmes, and P. P. Lee Microarray Analysis Reveals Differences in Gene Expression of Circulating CD8+ T Cells in Melanoma Patients and Healthy Donors Cancer Res., May 15, 2004; 64(10): 3661 - 3667. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. G. Martinez, E. H. Pellizzari, E. S. Diaz, S. B. Cigorraga, L. Lustig, B. Denduchis, C. Wolfenstein-Todel, and M. M. Iglesias Galectin-1, a cell adhesion modulator, induces apoptosis of rat Leydig cells in vitro Glycobiology, February 1, 2004; 14(2): 127 - 137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. V. Acosta-Rodriguez, C. L. Montes, C. C. Motran, E. I. Zuniga, F.-T. Liu, G. A. Rabinovich, and A. Gruppi Galectin-3 Mediates IL-4-Induced Survival and Differentiation of B Cells: Functional Cross-Talk and Implications during Trypanosoma cruzi Infection J. Immunol., January 1, 2004; 172(1): 493 - 502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Aandahl, J. K. Sandberg, K. P. Beckerman, K. Tasken, W. J. Moretto, and D. F. Nixon CD7 Is a Differentiation Marker That Identifies Multiple CD8 T Cell Effector Subsets J. Immunol., March 1, 2003; 170(5): 2349 - 2355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Correa, C. E. Sotomayor, M. P. Aoki, C. A. Maldonado, and G. A. Rabinovich Opposite effects of galectin-1 on alternative metabolic pathways of L-arginine in resident, inflammatory, and activated macrophages Glycobiology, February 1, 2003; 13(2): 119 - 128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Dettin, N. Rubinstein, A. Aoki, G. A. Rabinovich, and C. A. Maldonado Regulated Expression and Ultrastructural Localization of Galectin-1, a Proapoptotic {beta}-Galactoside-Binding Lectin, During Spermatogenesis in Rat Testis Biol Reprod, January 1, 2003; 68(1): 51 - 59. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Hernandez and L. G. Baum Ah, sweet mystery of death! Galectins and control of cell fate Glycobiology, October 1, 2002; 12(10): 127R - 136R. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. Rabinovich, N. Rubinstein, and L. Fainboim Unlocking the secrets of galectins: a challenge at the frontier of glyco-immunology J. Leukoc. Biol., May 1, 2002; 71(5): 741 - 752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Zuniga, C. C. Motran, C. L. Montes, H. Yagita, and A. Gruppi Trypanosoma cruzi Infection Selectively Renders Parasite-Specific IgG+ B Lymphocytes Susceptible to Fas/Fas Ligand-Mediated Fratricide J. Immunol., April 15, 2002; 168(8): 3965 - 3973. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Zuniga, A. Gruppi, J. Hirabayashi, K. I. Kasai, and G. A. Rabinovich Regulated Expression and Effect of Galectin-1 on Trypanosoma cruzi-Infected Macrophages: Modulation of Microbicidal Activity and Survival Infect. Immun., November 1, 2001; 69(11): 6804 - 6812. [Abstract] [Full Text] [PDF]
|
|
