Journal of Leukocyte Biology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kumar, R. K.
Right arrow Articles by Geczy, C. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kumar, R. K.
Right arrow Articles by Geczy, C. L.
(Journal of Leukocyte Biology. 2001;70:59-64.)
© 2001 by Society for Leukocyte Biology

Dimeric S100A8 in human neutrophils is diminished after phagocytosis

Rakesh K. Kumar*, Zheng Yang*, Susan Bilson*, Soula Thliveris*, Bridget E. Cooke{dagger} and Carolyn L. Geczy*

* School of Pathology, University of New South Wales, Sydney, Australia; and
{dagger} Department of Anatomical Pathology, Prince of Wales Hospital, Randwick, Australia

Correspondence: R. K. Kumar, School of Pathology, University of New South Wales, Sydney, Australia 2052. E-mail: R.Kumar{at}unsw.edu.au

S100A8 is a major cytoplasmic protein of neutrophils and monocytes/macrophages and has been associated with myeloid cell differentiation and activation. Little is known about its functions or mechanisms of release from neutrophils. We have developed a monoclonal antibody to murine S100A8, which cross-reacts with human S100A8. This antibody, which recognizes the homodimeric form of the protein, detects its expression specifically in human neutrophils and is reactive in formalin-fixed, paraffin-embedded tissues. Using this antibody as well as a commercially available antibody to human S100A8, we show that phagocytic activation of neutrophils, in vivo in acute appendicitis and in vitro following phagocytosis of opsonized zymosan, is characterized by loss of cytoplasmic immunoreactivity for S100A8. In vitro, phagocytosis is associated with rapid diminution of immunostaining without loss of viability. Loss of immunoreactivity for S100A8 may serve as a marker of localized neutrophil activation in tissues.

Key Words: acute inflammation • neutrophil activation • immuno-histochemistry • S100 proteins




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
C. Ryckman, C. Gilbert, R. de Medicis, A. Lussier, K. Vandal, and P. A. Tessier
Monosodium urate monohydrate crystals induce the release of the proinflammatory protein S100A8/A9 from neutrophils
J. Leukoc. Biol., August 1, 2004; 76(2): 433 - 440.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Berthier, M.-H. Paclet, S. Lerouge, F. Roux, S. Vergnaud, A. W. Coleman, and F. Morel
Changing the Conformation State of Cytochrome b558 Initiates NADPH Oxidase Activation: MRP8/MRP14 REGULATION
J. Biol. Chem., July 3, 2003; 278(28): 25499 - 25508.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. J. Raftery, Z. Yang, S. M. Valenzuela, and C. L. Geczy
Novel Intra- and Inter-molecular Sulfinamide Bonds in S100A8 Produced by Hypochlorite Oxidation
J. Biol. Chem., August 31, 2001; 276(36): 33393 - 33401.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the Society for Leukocyte Biology.