HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ibata-Ombetta, S. Articles by Poulain, D. Search for Related Content PubMed PubMed Citation Articles by Ibata-Ombetta, S. Articles by Poulain, D.

(Journal of Leukocyte Biology. 2001;70:149-154.)
© 2001 by Society for Leukocyte Biology

Role of extracellular signal-regulated protein kinase cascade in macrophage killing of Candida albicans

Laboratoire de Mycologie Fondamentale et Appliquée, INSERM EPI 9915, Université de Lille II, Faculté de Médecine H. Warembourg, Pôle Recherche, 59037 Lille Cedex, France

Correspondence: Thierry Jouault, Laboratoire de Mycologie Fondamentale et appliquée, Université de Lille II, Faculté de Médecine H. Warembourg, Pôle Recherche, Place Verdun, 59037 Lille Cedex, France. E-mail: tjouault{at}univ-lille2.fr

The pathogenic yeast Candida albicans and its derived molecules stimulate a wide range of macrophage secretory functions and may adapt to escape being killed by this phagocyte. In this study, phagocytosis of C. albicans and of the nonpathogenic yeast Saccharomyces cerevisiae was shown to be associated with phosphorylation of the mitogen-activated protein kinase (MAPK)/extracellularly regulated kinase (ERK) pathway in the absence of significant activation of either p38MAPK or stress-activated protein kinase/c-Jun N-terminal kinase. However, although 80% of endocytosed C. albicans survived after 1 h, 80% of S. cerevisiae cells were killed. Considerable quantitative differences were observed between the two species in the sequential phosphorylation of MAPK/ERK kinase (MEK), extracellularly regulated kinase-1, and 90-kDa-ribosomal S6 kinases. A lower level of activation of the pathway by C. albicans was associated with a species-specific overexpression of the MEK phosphatase MAPK phosphatase (MKP)-1. Killing of both C. albicans and S. cerevisiae could be reduced using PD98059, which mimics MKP-1 and inhibits MEK phosphorylation, suggesting that specific MKP-1 activation by C. albicans could contribute to its ability to escape the yeast lytic potential of macrophages.

Key Words: MAP kinases • phagocytosis • yeast • pathogenicity • macrophages

This article has been cited by other articles:

				M. Dubourdeau, R. Athman, V. Balloy, M. Huerre, M. Chignard, D. J. Philpott, J.-P. Latge, and O. Ibrahim-Granet Aspergillus fumigatus Induces Innate Immune Responses in Alveolar Macrophages through the MAPK Pathway Independently of TLR2 and TLR4 J. Immunol., September 15, 2006; 177(6): 3994 - 4001. [Abstract] [Full Text] [PDF]

				F. Navarro-Garcia, B. Eisman, S. M. Fiuza, C. Nombela, and J. Pla The MAP kinase Mkc1p is activated under different stress conditions in Candida albicans Microbiology, August 1, 2005; 151(8): 2737 - 2749. [Abstract] [Full Text] [PDF]

				C. Du, R. Calderone, J. Richert, and D. Li Deletion of the SSK1 Response Regulator Gene in Candida albicans Contributes to Enhanced Killing by Human Polymorphonuclear Neutrophils Infect. Immun., February 1, 2005; 73(2): 865 - 871. [Abstract] [Full Text] [PDF]

				C. Mille, G. Janbon, F. Delplace, S. Ibata-Ombetta, C. Gaillardin, G. Strecker, T. Jouault, P.-A. Trinel, and D. Poulain Inactivation of CaMIT1 Inhibits Candida albicans Phospholipomannan {beta}-Mannosylation, Reduces Virulence, and Alters Cell Wall Protein {beta}-Mannosylation J. Biol. Chem., November 12, 2004; 279(46): 47952 - 47960. [Abstract] [Full Text] [PDF]

				K. Nakamichi, S. Inoue, T. Takasaki, K. Morimoto, and I. Kurane Rabies Virus Stimulates Nitric Oxide Production and CXC Chemokine Ligand 10 Expression in Macrophages through Activation of Extracellular Signal-Regulated Kinases 1 and 2 J. Virol., September 1, 2004; 78(17): 9376 - 9388. [Abstract] [Full Text] [PDF]

				H. Shiratsuchi and M. D. Basson Extracellular pressure stimulates macrophage phagocytosis by inhibiting a pathway involving FAK and ERK Am J Physiol Cell Physiol, June 1, 2004; 286(6): C1358 - C1366. [Abstract] [Full Text] [PDF]

				S. IBATA-OMBETTA, T. IDZIOREK, P.-A. TRINEL, D. POULAIN, and T. JOUAULT Role of Phospholipomannan in Candida albicans Escape from Macrophages and Induction of Cell Apoptosis through Regulation of Bad Phosphorylation Ann. N.Y. Acad. Sci., December 1, 2003; 1010(1): 573 - 576. [Abstract] [Full Text] [PDF]

				B. Zhang, J. Hirahashi, X. Cullere, and T. N. Mayadas Elucidation of Molecular Events Leading to Neutrophil Apoptosis following Phagocytosis: CROSS-TALK BETWEEN CASPASE 8, REACTIVE OXYGEN SPECIES, AND MAPK/ERK ACTIVATION J. Biol. Chem., August 1, 2003; 278(31): 28443 - 28454. [Abstract] [Full Text] [PDF]

				S. Ibata-Ombetta, T. Idziorek, P.-A. Trinel, D. Poulain, and T. Jouault Candida albicans Phospholipomannan Promotes Survival of Phagocytosed Yeasts through Modulation of Bad Phosphorylation and Macrophage Apoptosis J. Biol. Chem., April 4, 2003; 278(15): 13086 - 13093. [Abstract] [Full Text] [PDF]

				G. Newport, A. Kuo, A. Flattery, C. Gill, J. J. Blake, M. B. Kurtz, G. K. Abruzzo, and N. Agabian Inactivation of Kex2p Diminishes the Virulence of Candida albicans J. Biol. Chem., January 10, 2003; 278(3): 1713 - 1720. [Abstract] [Full Text] [PDF]