* Department of Allergy and Rheumatology,
Department of Molecular Preventive Medicine and CREST,
|| Department of Respiratory Medicine, and
** Department of Bioregulatory Function, University of Tokyo Graduate School of Medicine, Tokyo, Japan;
Department of Clinical Immunology and AIDS Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan;
Shionogi Institute for Medical Science, Osaka, Japan; and
# Department of Bacteriology, Kinki University, Osaka, Japan
Correspondence: Koichi Hirai, M.D., Ph.D., Department of Bioregulatory Function, University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan. E-mail: hiraiko-tky{at}umin.ac.jp
We examined the expression profile of chemokine receptors in human basophils and their regulation by cytokines. Basophils expressed transcripts of CC chemokine receptors (CCR)1, CCR2, CCR3, and CCR5 and CXC chemokine receptors (CXCR)1, CXCR2, and CXCR4. In contrast to the other receptors, surface-CXCR4 expression was not detected in fresh- and whole-blood basophils, but it became apparent gradually during incubation. Among 16 chemokines tested, eotaxin induced the most potent basophil migration. SDF-1 also induced a strong, migratory response comparable with that induced by eotaxin in 24-h, cultured basophils, but it failed to induce degranulation. IL-3 abrogated CXCR4 expression completely, and it only down-regulated CCR2 and CCR3 expression slightly. IL-5, GM-CSF, and IL-4 also down-regulated CXCR4 expression. Thus, expression of CXCR4 was the most strongly affected by cytokines, and this may represent an alternative mechanism for control of cell-specific, biological responses to SDF-1.
Key Words: SDF-1 • CCR5 • migration
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