Accuri C6 Flow Cytometer System

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(Journal of Leukocyte Biology. 2001;69:995-1005.)
© 2001 by Society for Leukocyte Biology

Expression, tissue distribution, and cellular localization of the antiapoptotic TIP-B1 protein

Erica S. Berleth, Patricia A. Masso-Welch, Latif A. Kazim, Margot M. Ip, Enrico Mihich and M. Jane Ehrke

Department of Pharmacology and Therapeutics, Grace Cancer Drug Center, Roswell Park Cancer Institute, Buffalo, New York

Correspondence: Dr. Erica Berleth, Grace Cancer Drug Center, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263. E-mail: erica.berleth{at}roswellpark.org

TIP-B1 is a novel 27-kDa protein isolated from the cytosol of tumor necrosis factor (TNF)-stimulated cells. Cells preincubated with TIP-B1 are protected from TNF-induced apoptosis. This study showed that, as with normal fibroblasts and U937 histiocytic lymphoma, human MCF7 mammary adenocarcinoma cells were protected from TNF in a concentration-dependent manner by pretreatment with either TNF or purified TIP-B1. Immunoblot and immunohistochemical analyses indicated expression of both TIP-B1 mRNA and protein in MCF7 cells and heart, kidney, brain, liver, ovary, uterus, thymus, spleen, lymph node, and mammary gland cells throughout their development. Expression of TIP-B1 was heterogeneous, with staining of specific cell types within tissues. Based on the ability of TIP-B1 to protect both normal and tumor cells from TNF-induced apoptosis and its broad tissue distribution, with expression only in select cells within those tissues, a role for TIP-B1 in the regulation of TNF-induced effects is strongly indicated.

Key Words: mammary • apoptosis • TNF • lymphoid • T cell