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(Journal of Leukocyte Biology. 2001;69:969-976.)
© 2001 by Society for Leukocyte Biology

Differences in splenic B-lymphocyte ganglioside expression and accessibility in normal and endotoxin-hyporesponsive mice

Charles S. Berenson*, Robin H. Rasp*, Jen-Tzer Gau*, John L. Ryan{dagger} and Herbert C. Yohe{ddagger},§

* Infectious Diseases Section, Department of Veterans Affairs Western New York Healthcare System and State University of New York at Buffalo, Buffalo, New York;
{dagger} Genetics Institute, Cambridge, Massachusetts;
{ddagger} Department of Veterans Affairs, White River Junction, Vermont; and
§ Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, New Hampshire

Correspondence: Charles S. Berenson, Infectious Diseases Laboratory-151, Buffalo V.A. Medical Center, Buffalo, NY 14215. E-mail: berenson{at}acsu.buffalo.edu

Endotoxin-responsive (C3H/HeN) and -hyporesponsive (C3H/HeJ) murine B lymphocytes purified by adherence to anti-immunoglobulin ("antibody panning") possess identical gangliosides but different ganglioside surface accessibilities. We investigated the distribution and surface accessibility of gangliosides of B lymphocytes purified by adherence to plastic ("plastic panning") or by subtraction of non-B-lymphocyte components. As with antibody panning, there were no entirely new or absent gangliosides in plastic-panned or subtraction-purified B lymphocytes of each strain. However, striking changes in relative expression of five gangliosides were detected with each purification protocol. Moreover, five gangliosides of antibody-panned and plastic-panned B lymphocytes but only two gangliosides of subtraction-purified B lymphocytes were inaccessible to surface labeling. Unlike the situation for antibody-panned B lymphocytes, no interstrain (HeN vs. HeJ) surface accessibility differences existed in gangliosides of plastic-panned or subtraction-purified cells. Exposure of subtraction-purified B lymphocytes to anti-immunoglobulin failed to elicit changes in ganglioside expression. Murine B lymphocytes have distinct protocol-dependent differences in glycolipid phenotype which likely denote individual subpopulations.

Key Words: glycosphingolipids • surface accessibility • immune cells • thin-layer chromatography




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C. S. Berenson, M. A. Gallery, J. M. Smigiera, and R. H. Rasp
The role of ceramide of human macrophage gangliosides in activation of human macrophages
J. Leukoc. Biol., September 1, 2002; 72(3): 492 - 502.
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