Accuri C6 Flow Cytometer System

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(Journal of Leukocyte Biology. 2001;69:944-950.)
© 2001 by Society for Leukocyte Biology

Tissue distribution of CD6 and CD6 ligand in cattle: expression of the CD6 ligand (CD166) in the autonomic nervous system of cattle and the human

A. Konno*, J.-S. Ahn{dagger}, H. Kitamura*, M. J. Hamilton{dagger}, J. A. Gebe{ddagger}, A. Aruffo{ddagger} and W. C. Davis{dagger}

* Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan;
{dagger} Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington; and
{ddagger} Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey

Correspondence: Akihiro Konno, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan. E-mail: akonno{at}vetmed.hokudai.ac.jp

We studied the tissue distribution of CD6+ lymphocytes and cells expressing the CD6 ligand (also known as activated leukocyte cell adhesion molecule CD166) in calves by immunohistochemistry using an anti-bovine CD6 monoclonal antibody (mAb), a human CD6 (huCD6)-immunoglobulin G1 fusion protein (huCD6-Ig), and an anti-human CD166 (anti-huCD166) mAb. The huCD6-Ig and anti-huCD166 mAb bound to the sympathetic and parasympathetic nerve fibers but not to myelinated nerve fibers in the spinal nerve. Studies with human tissue using the anti-huCD166 mAb yielded identical patterns of labeling. Dense accumulations of CD6+ lymphocytes were present in areas of the thymuses and spleens of calves, in areas innervated by huCD6-Ig+ nerves. The cDNAs encoding the bovine CD166 and CD6 were isolated from the sympathetic ganglion and spleen, respectively. Predicted amino acid residues that are important for human and mouse CD6-CD166 binding were also conserved in bovine CD6 and CD166. Bovine CD166 transcripts were detected by reverse transcriptase-PCR in all the tissues that bound huCD6-Ig. These results show that the bovine orthologue of CD166 was constitutively expressed in the autonomic nervous systems of cattle and suggest that CD6+ lymphocytes adhere to CD166+ autonomic nerve terminals via CD6.

Key Words: bovine • {gamma}{delta} T cell • adhesion molecule • sympathetic nerve • parasympathetic nerve




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