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(Journal of Leukocyte Biology. 2001;69:937-943.)
© 2001 by Society for Leukocyte Biology

Transient expansion of peptide-specific lymphocytes producing IFN- after vaccination with dendritic cells pulsed with MAGE peptides in patients with mage-A1/A3-positive tumors

M. Toungouz^*, M. Libin^*, F. Bulté^*, L. Faid, F. Lehmann, D. Duriau, M. Laporte, D. Gangji, C. Bruyns, M. Lambermont^*, M. Goldman^|| and T. Velu^,

^* Unité de Thérapie Cellulaire et Moléculaire (U.T.C.M.),
Department of Medical Oncology,
Department of Dermatology,
Interdisciplinary Research Institute (IRIBHN), and
^|| Department of Immuno-Hematology-Tranfusion, Erasme Hospital and Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium

Correspondence: Dr. M. Toungouz, U.T.C.M., Erasme Hospital, 808 route de Lennik, B-1070 Brussels, Belgium. E-mail: toungouz{at}ulb.ac.be

Assessment of T-cell activation is pivotal for evaluation of cancer immunotherapy. We initiated a clinical trial in patients with MAGE-A1 and/or -A3 tumors using autologous DC pulsed with MAGE peptides aimed at analyzing T-cell-derived, IFN- secretion by cytokine flow cytometry and ELISPOT. We also tested whether further KLH addition could influence this response favorably. Monocyte-derived DC were generated from leukapheresis products. They were pulsed with the relevant MAGE peptide(s) alone in group A (n=10 pts) and additionally with KLH in group B (n=16 pts). A specific but transient increase in the number of peripheral blood T lymphocytes secreting IFN- in response to the vaccine peptide(s) was observed in 6/8 patients of group A and in 6/16 patients of group B. We conclude that anti-tumor vaccination using DC pulsed with MAGE peptides induces a potent but transient anti-MAGE, IFN- secretion that is not influenced by the additional delivery of a nonspecific, T-cell help.

Key Words: T-cell response • keyhole limpet hemocyanin • ELISPOT • cytokine flow cytometry

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