PeproTech Inc.

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jan, T.-R.
Right arrow Articles by Kaminski, N. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jan, T.-R.
Right arrow Articles by Kaminski, N. E.
(Journal of Leukocyte Biology. 2001;69:841-849.)
© 2001 by Society for Leukocyte Biology

Role of mitogen-activated protein kinases in the differential regulation of interleukin-2 by cannabinol

Tong-Rong Jan and Norbert E. Kaminski

Department of Pharmacology and Toxicology. Michigan State University, East Lansing, Michigan

Correspondence: Norbert E. Kaminski, Ph.D., Department of Pharmacology and Toxicology, 315 Food Safety and Toxicology Building, Michigan State University, East Lansing, MI 48824. E-mail: kamins11{at}pilot.msu.edu

Cannabinoids can paradoxically regulate interleukin-2 (IL-2) expression either positively or negatively. This study investigated the mechanism responsible for cannabinol-mediated IL-2 modulation. In primary murine splenocytes and EL4.IL-2 T cells, the contrasting effects of cannabinol on IL-2 secretion depended on the magnitude but not the mode of T-cell activation. Suboptimal activation of T cells in the presence of cannabinol produced an enhancement of IL-2 secretion, which was paralleled by an increase in nuclear phospho-extracellular-regulated kinase (ERK) 1/2. In contrast, T cells activated with stimuli that were optimized to induce maximal IL-2 secretion elicited a marked suppression in the production of this cytokine when cultured in the presence of cannabinol. Moreover, cannabinol-mediated enhancement of IL-2 secretion by splenocytes was attenuated to various degrees by staurosporine, Ro-31-8220, and KN93. These results suggest that the enhancement of IL-2 secretion by cannabinol is associated with an increase in ERK mitogen-activated protein kinase, which is protein kinase C and calmodulin-kinase dependent.

Key Words: Cannabinol • Interleukin-2 • Mitogen-activated protein kinase • Protein kinase C • Calcium/calmodulin-dependent protein kinases • T lymphocyte.




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
H. Lu, B. L. F. Kaplan, T. Ngaotepprutaram, and N. E. Kaminski
Suppression of T cell costimulator ICOS by {Delta}9-tetrahydrocannabinol
J. Leukoc. Biol., February 1, 2009; 85(2): 322 - 329.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
B. L. F. Kaplan, C. E. Rockwell, and N. E. Kaminski
Evidence for Cannabinoid Receptor-Dependent and -Independent Mechanisms of Action in Leukocytes
J. Pharmacol. Exp. Ther., September 1, 2003; 306(3): 1077 - 1085.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
T.-R. Jan, G. K. Rao, and N. E. Kaminski
Cannabinol Enhancement of Interleukin-2 (IL-2) Expression by T Cells Is Associated with an Increase in IL-2 Distal Nuclear Factor of Activated T Cell Activity
Mol. Pharmacol., February 1, 2002; 61(2): 446 - 454.
[Abstract] [Full Text] [PDF]