Unité de Recherche en Pneumologie, Centre de Recherche, Hôpital Laval, Institut Universitaire de Cardiologie et Pneumologie de l’Université, Laval, Quebec, Canada
Correspondence: Michel Laviolette, Hôpital Laval, 2725 Chemin Sainte-Foy, Sainte-Foy, Quebec G1V 4G5, Canada.. E-mail: medmla{at}hermes.ulaval.ca
The effect of eotaxin, a potent eosinophil chemotactic factor, on
eosinophil transmigration through a reconstituted basal membrane
(Matrigel®) was evaluated. Eotaxin induced significant eosinophil
transmigration in the presence of 10% fetal bovine serum (FBS) and
interleukin-5. Its effect was optimal at 0.01 µM, and it plateaued at
18 h. Eotaxin’s effect was greater with eosinophils from
asthmatic subjects (61.1 ± 3.4%) than with eosinophils from
normal subjects (38.7 ± 4.2%) (P < 0.001).
Inhibition of metalloproteinases decreased eotaxin-induced
transmigration by 
10.4%, whereas inhibition of the
plasminogen-plasmin system decreased eotaxin’s effect by 44.4%
(P = 0.0002). Moreover, eotaxin-induced transmigration
was largely diminished in medium with low concentrations of serum
[0.5% FBS: 6.1 ± 2.4%; 10% FBS: 40.2 ± 5.8%
(P = 0.0001)] but returned to its initial level with
the addition of plasminogen (2 U/mL) to 0.5% FBS (43.1 ± 6.5%).
These data show that eotaxin is an efficient promoter of eosinophil
transmigration in vitro, that it is more potent with cells
from asthmatics than with normal cells, and that its effect depends
predominantly on the activation of the plasminogen-plasmin
system.
Key Words: asthma • 5-oxo-ETE • metalloproteinases • urokinase plasminogen activator receptor
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