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(Journal of Leukocyte Biology. 2001;69:747-754.)
© 2001 by Society for Leukocyte Biology

Changes of HSP72-expression in leukocytes are associated with adaptation to exercise under conditions of high environmental temperature

Elvira Fehrenbach^*, Andreas Michael Niess, Roman Veith^*, Hans-Herrmann Dickhuth and Hinnak Northoff^*

^* Department of Transfusion Medicine, and
Medical Clinic and Polyclinic, Department of Sports Medicine, University of Tuebingen, Tuebingen, Germany

Correspondence: Elvira Fehrenbach, Ph.D., Abteilung Transfusionsmedizin, Eberhard-Karls-Universitaet Tuebingen, Otfried-Mueller-Str. 4/1, D-72076 Tuebingen, Germany. E-mail: elvira.fehrenbach{at}med.uni-tuebingen.de

Overexpression of the heat shock protein HSP72 provides thermotolerance. We asked if two consecutive endurance runs 1 week apart (CR1, CR2) and additional environmental heat stress affect HSP72-expression in leukocytes of nonheat-acclimated endurance athletes. Twelve subjects were allocated randomly into two groups. Group HH completed both runs at 28°C ambient temperature, and group NH performed CR1 at 18°C and CR2 at 28°C. HSP72-expression was determined by flow cytometry and RT-PCR before and 0, 24, and 48 h after exercise. Additionally, post-exercise cells were exposed to in vitro heat shock (HS; 2 h, 42°C). The prolonged, high HSP72 protein level after CR1 in HH compared with NH may reflect thermotolerance induced by endurance exercise at high ambient temperature. Adaptation of cardiocirculatory/thermoregulatory capacity after CR2 in HH went along with a more rapid down-regulation of HSP72 compared with CR1. HSP72 mRNA demonstrated temperature-related changes after exercise. The reduced HS response in vitro after CR2 may represent exercise-related adaptation mechanisms. HSP72 concentrations in leukocytes may indicate previous exercise- and temperature-related stress conditions and adaptation in immunocompetent cells.

Key Words: heat shock protein • hyperthermia • acclimatization • thermotolerance • in vitro

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