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(Journal of Leukocyte Biology. 2001;69:645-650.)
© 2001 by Society for Leukocyte Biology

Expression of the apolipoprotein C-II gene during myelomonocytic differentiation of human leukemic cells

Eun Mi Chun, Young Jae Park, Hong Soon Kang, Hyun Min Cho, Do Youn Jun and Young Ho Kim

Department of Microbiology, College of Natural Sciences, Kyungpook National University, Taegu 702-701, Korea

Correspondence: Young Ho Kim, Department of Microbiology, College of Natural Sciences, Kyungpook National University, Taegu 702-701, Korea. E-mail: ykim{at}kyungpook.ac.kr

Apolipoprotein C-II (apoC-II), which is known to activate lipoprotein lipase (LPL), was identified by ordered differential display (ODD)-polymerase chain reaction (PCR) as a cDNA fragment exhibiting a distinct increase in expression during 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced differentiation of promonocytic U937 cells into monocytes and macrophages. The amount of apoC-II mRNA expression detectable in U937 cells significantly increased and reached a maximum 24–48 h after treatment with 32 nM TPA. apoC-II mRNA was also detected in monocytic THP-1 cells but was not detected in promyelocytic HL-60 cells. In healthy human tissues, the most significant expression of apoC-II mRNA was in the liver. Although apoC-II mRNA expression was markedly up-regulated during the induced differentiation of HL-60 cells into monocytes and macrophages with 32 nM TPA, such expression was not induced during the differentiation of HL-60 cells into granulocytes with 1.25% dimethyl sulfoxide. These results suggest that human apoC-II expression is induced at the transcription level during myelomonocytic differentiation and may confer an important role to macrophages involved in normal lipid metabolism and atherosclerosis.

Key Words: ODD-PCR • macrophage • cell differentiation




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