Institute of Cancer Research and Molecular Biology, The Norwegian University of Science and Technology, N-7489 Trondheim, Norway
Human Toll-like receptor 2 (TLR2) is a receptor for a variety of
microbial products and mediates activation signals in cells of the
innate immune system. We have investigated expression and regulation of
the TLR2 protein in human blood cells and tissues by using two
anti-TLR2 mAbs. Only myelomonocytic cell lines expressed surface TLR2.
In tonsils, lymph nodes, and appendices, activated B-cells in germinal
centers expressed TLR2. In human blood, CD14+ monocytes
expressed the highest level of TLR2 followed by CD15+
granulocytes, and CD19+ B-cells, CD3+ T-cells,
and CD56+ NK cells did not express TLR2. The level of TLR2
on monocytes was after 20 h up-regulated by LPS, GM-CSF, IL-1, and
IL-10 and down-regulated by IL-4, IFN-
, and TNF. On purified
granulocytes, LPS, GM-CSF, and TNF down-regulated, and IL-10 modestly
increased TLR2 expression after 2 h. These data suggest that TLR2
protein expression in innate immune cells is differentially regulated
by inflammatory mediators.
Key Words: monocytes • granulocytes • innate immunity cells
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