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(Journal of Leukocyte Biology. 2001;69:419-425.)
© 2001 by Society for Leukocyte Biology

A p74 common gamma receptor chain isoform facilitates IL-2 and IL-15 responses by the myelomonocytic cell line Tf-1ß2

Nancy L. Monson^*, Timothy J. Fenske, Shyng Wei^#, Angela J. Okragly^**, Jill L.O. de Jong^*, Mary Haak-Frendscho^**, John O’Shea^||, Julie Djeu^# and Paul M. Sondel^*,#,**

Departments of
^* Human Oncology,
Molecular and Cellular Biology,
^# Pediatrics, and
^** Genetics, University of Wisconsin, and University of Wisconsin Comprehensive Cancer Center, Madison, Wisconsin;
H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida;
Promega Corp., Madison, Wisconsin; and
^|| Arthritis and Rheumatoid Branch, National Institutes of Health, Bethesda, Maryland

Correspondence: Nancy L. Monson, Ph.D., Department of Neurology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9036. E-mail: nancy.monson{at}utsouthwestern.edu

Functional forms of the IL-2, IL-4, IL-7, IL-9, and IL-15 receptors require the c receptor component. We have described previously a myeloid cell line called Tf-1ß, which binds IL-2 with intermediate-affinity and proliferates in response to IL-2. In this study, we characterize c expression on Tf-1ß2 cells, a derivative of Tf-1ß cells stimulated exclusively with IL-2. Although Tf-1ß2 cells bind IL-2 with intermediate-affinity and proliferate in response to IL-2, this cell line does not express the p64 c chain at the protein level. This result was surprising because prior studies suggest these cells should not be expected to proliferate in response to IL-2 or IL-15 in the absence of the p64 c chain. A p74 protein was detected by western blot following immunoprecipitation with an anti-c polyclonal antibody, and a p74 protein was identified consistently in complex with IL-2 and IL-15 on these cells. However, the c gene in these Tf-1ß2 cells shows no evidence of mutation by sequence analysis. Furthermore, inhibition of glycosylation of these Tf-1ß2 cells by tunicamycin treatment yields a standard 39-kDa molecule recognized on western blot with anti-c antibody, as seen for the standard 64-kDa isoform of c. These results demonstrate that a 74-kDa c receptor isoform was involved in the response of the Tf-1ß2 cells to cytokines which normally interact with the 64-kDa c chain.

Key Words: monocytes • cytokines • cytokine receptors • signaling