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* Immunopharmacology Laboratory, Department of Biochemistry and Immunology, and
Department of Pathology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;
Department of Pathology, University of Michigan, Ann Arbor, Michigan; and
Cardiovascular Research Group, University of Sheffield, United Kingdom
Correspondence: Mauro Martins Teixeira, M.D., Ph.D., Immunopharmacology, Departamento de Bioquímica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Av. Antonio Carlos, 6627—Pampulha, 31270-901 Belo Horizonte MG Brasil. E-mail: mmtex{at}icb.ufmg.br
The selective accumulation of eosinophils in tissue is a characteristic
feature of allergic diseases where there is a predominance of
lymphocytes expressing a Th2 phenotype. In an attempt to define factors
determining specific eosinophil accumulation in vivo, we
have used a radiolabeled technique to assess the occurrence and the
mechanisms underlying 111In-eosinophil recruitment into
Th1- and Th2-predominant, delayed-type hypersensitivity (DTH)
reactions. Eosinophils were purified from the blood of IL-5 transgenic
mice, labeled with 111In and injected into nontransgenic
CBA/Ca mice. Th1- and Th2-predominant, DTH reactions were induced in
mice by immunization with methylated bovine serum albumin (MBSA) in
Freund’s complete adjuvant or with Schistosoma mansoni
eggs, respectively. In these animals, 111In-eosinophils
were recruited in skin sites in an antigen-, time-, and
concentration-dependent manner. Depletion of CD4+
lymphocytes abrogated 111In-eosinophil recruitment in both
reactions. Pretreatment of animals with anti-IFN-
mAb abrogated
111In-eosinophil recruitment in MBSA-immunized and
-challenged animals, whereas anti-IL-4 inhibited
111In-eosinophil recruitment in both models. Local
pretreatment with an anti-eotaxin polyclonal antibody inhibited the
MBSA and SEA reactions by 51% and 39%, respectively. These results
demonstrate that, although eosinophilia is not a feature of
Th1-predominant, DTH reactions, these reactions produce the necessary
chemoattractants and express the necessary cell adhesion molecules for
eosinophil migration. The control of the circulating levels of
eosinophils appears to be a most important strategy in determining
tissue eosinophilia.
Key Words: chemokines • bone marrow • interleukin-4 • lymphocytes
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