HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hope, J. C. Articles by Howard, C. J. Search for Related Content PubMed PubMed Citation Articles by Hope, J. C. Articles by Howard, C. J.

(Journal of Leukocyte Biology. 2001;69:271-279.)
© 2001 by Society for Leukocyte Biology

Differences in the induction of CD8⁺ T cell responses by subpopulations of dendritic cells from afferent lymph are related to IL-1 secretion

Institute for Animal Health, Compton, Newbury, Berkshire, United Kingdom

Correspondence: Dr. J. C. Hope, Institute for Animal Health, Compton, Newbury, Berkshire, RG20 7NN, UK. E-mail: Jayne.Hope{at}BBSRC.ac.uk

The major subset of dendritic cells (DC) from bovine afferent lymph expresses the SIRP MyD-1 antigen, but not CD11a or the antigen recognized by mAb CC81, and potently stimulates CD4⁺ and CD8⁺ T lymphocyte proliferation. The minor subpopulation, that is CD11a⁺CC81⁺MyD-1^-, effectively stimulates CD4⁺ but not CD8⁺ T lymphocyte proliferation. CD11a⁺CC81⁺MyD-1^- DC did not induce anergy or death or secrete an inhibitory factor. However, supernatant from cultures of CD8⁺ T cells with CD11a^-CC81^-MyD-1⁺ DC significantly enhanced proliferation of CD8⁺ T cells in response to CD11a⁺CC81⁺MyD-1^- DC, an effect that was blocked by interleukin (IL)-1, but not IL-1ß, specific mAb. The proliferation of CD8⁺ T cells with CD11a⁺CC81⁺MyD-1^- DC was also enhanced by adding IL-1. IL-1ß slightly enhanced proliferation, whereas IL-2, IL-6, IL-12, and IL-15 had no effect. We conclude that the failure to stimulate CD8⁺ T cell proliferation results from the lack of IL-1 synthesis by this population, which may have important consequences in vivo.

Key Words: afferent lymph veiled cells • CD8⁺ lymphocytes

This article has been cited by other articles:

				A. Nambu, S. Nakae, and Y. Iwakura IL-1{beta}, but not IL-1{alpha}, is required for antigen-specific T cell activation and the induction of local inflammation in the delayed-type hypersensitivity responses Int. Immunol., May 1, 2006; 18(5): 701 - 712. [Abstract] [Full Text] [PDF]

				Y. Liu, I. Soto, Q. Tong, A. Chin, H.-J. Buhring, T. Wu, K. Zen, and C. A. Parkos SIRP{beta}1 Is Expressed as a Disulfide-linked Homodimer in Leukocytes and Positively Regulates Neutrophil Transepithelial Migration J. Biol. Chem., October 28, 2005; 280(43): 36132 - 36140. [Abstract] [Full Text] [PDF]

				M. Epardaud, M. Bonneau, F. Payot, C. Cordier, J. Megret, C. Howard, and I. Schwartz-Cornil Enrichment for a CD26hi SIRP- subset in lymph dendritic cells from the upper aero-digestive tract J. Leukoc. Biol., September 1, 2004; 76(3): 553 - 561. [Abstract] [Full Text] [PDF]

				A. A. Bajer, D. Garcia-Tapia, K. R. Jordan, K. M. Haas, D. Werling, C. J. Howard, and D. M. Estes Peripheral blood-derived bovine dendritic cells promote IgG1-restricted B cell responses in vitro J. Leukoc. Biol., January 1, 2003; 73(1): 100 - 106. [Abstract] [Full Text] [PDF]

				C. Voisine, F.-X. Hubert, B. Trinite, M. Heslan, and R. Josien Two Phenotypically Distinct Subsets of Spleen Dendritic Cells in Rats Exhibit Different Cytokine Production and T Cell Stimulatory Activity J. Immunol., September 1, 2002; 169(5): 2284 - 2291. [Abstract] [Full Text] [PDF]