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(Journal of Leukocyte Biology. 2001;69:215-223.)
© 2001 by Society for Leukocyte Biology

Antibodies recognizing CD24 LAP epitope on human T cells enhance CD28 and IL-2 T cell proliferation

María del C. Salamone, Carolina Rosselot, Gabriela V. Salamone, Marcos Barboza, Miguel Kado and Leonardo Fainboim

Immunogenetic Division, University Hospital, School of Medicine, University of Buenos Aires, Argentina

Correspondence: María del Carmen Salamone, Ph.D., División Immunogenética, Hospital de Clínicas, José de San Martín, Av Córdoba 2351, 3° Piso, (1120) Buenos Aires, Argentina. E-mail: marysasinectis{at}com.ar

Membrane expression of the CD24 molecule on activated T lymphocytes is not elucidated fully. We previously described the intracellular and cell-surface expression of the CD24 sialic acid-dependent epitope(s) on phytohemagglutinin-activated peripheral blood mononuclear cells. However, the CD24 core protein was not detected previously on human T cells. This study reinvestigated the expression and role of CD24 in T cell subsets. We analyzed binding of anti-CD24 monoclonal antibodies (mAbs) to sialic and leucine-alanine-proline (LAP) epitopes in resting and activated, normal T lymphocytes. CD24 LAP and CD24 sialic epitopes were detected on activated CD4- and CD8-positive cells. Although expression of CD24 sialic epitopes remained stably expressed in interleukin (IL)-2-dependent cultures, T cell expression of the LAP epitope was transient. Anti-LAP antibodies strongly enhanced the response of T cells to a combination of anti-CD3/CD28 mAbs and enhanced proliferative response induced by recombinant IL-2. We found similarities in the tissue distribution and function of the human CD24 LAP molecule and the murine, heat-stable antigen, which suggests that CD24 might function as a signaling molecule on human T cells.

Key Words: CD24 antigen • costimulation signals • PBMC • phytohemagglutinin






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