Accuri C6 Flow Cytometer System

This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Getting, S. J.
Right arrow Articles by Perretti, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Getting, S. J.
Right arrow Articles by Perretti, M.
(Journal of Leukocyte Biology. 2001;69:98-104.)
© 2001 by Society for Leukocyte Biology

Natural and synthetic agonists of the melanocortin receptor type 3 possess anti-inflammatory properties

Stephen J. Getting, Graham H. Allcock, Roderick Flower and Mauro Perretti

The William Harvey Research Institute, Charterhouse Square, London, England

Correspondence: Stephen J. Getting, Ph.D., Department of Biochemical Pharmacology, The William Harvey Research Institute, Charterhouse Square, London EC1M 6BQ, England. E-mail: S.J.Getting{at}mds.qmw.ac.uk

The effects of the natural and synthetic ligands for the melanocortin receptor type 3 (MC3-R) have been evaluated in a murine model of experimental gout. Systemic treatment of mice with {gamma}2-melanocyte-stimulating hormone ({gamma}2-MSH) and the synthetic agonist MTII inhibited accumulation of KC, interleukin-1 beta (IL-1ß), and PMN elicited by urate crystals in the peritoneal cavity. In vitro, macrophage (Mø) activation, determined as release of KC and IL-1ß, was inhibited by {gamma}2-MSH and MTII. The mixed MC3/4-R antagonist SHU9119 prevented the inhibitory actions of {gamma}2-MSH and MTII in vitro and in vivo, whereas the selective MC4-R antagonist HS024 was without effect. Western blotting also showed the presence of MC3-R protein on murine peritoneal Mø. Furthermore, agonism at the MC3-R evoked accumulation of cAMP within the Mø, which was inhibited by SHU9119. Thus, naturally occurring melanocortins, as well as the synthetic long-acting compound MTII, activate MC3-R on peritoneal Mø to inhibit the experimental inflammatory response.

Key Words: {gamma}2-MSH • MTII • KC • IL-1ß • inflammation




This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
G. S. Cooke, S. J. Campbell, S. Bennett, C. Lienhardt, K. P. W. J. McAdam, G. Sirugo, O. Sow, P. Gustafson, F. Mwangulu, P. van Helden, et al.
Mapping of a Novel Susceptibility Locus Suggests a Role for MC3R and CTSZ in Human Tuberculosis
Am. J. Respir. Crit. Care Med., July 15, 2008; 178(2): 203 - 207.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
A. Catania
The melanocortin system in leukocyte biology
J. Leukoc. Biol., February 1, 2007; 81(2): 383 - 392.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
S. J. Getting, C. W. Lam, G. Leoni, F. N. E. Gavins, P. Grieco, and M. Perretti
[D-Trp8]-{gamma}-Melanocyte-Stimulating Hormone Exhibits Anti-Inflammatory Efficacy in Mice Bearing a Nonfunctional MC1R (Recessive Yellow e/e Mouse)
Mol. Pharmacol., December 1, 2006; 70(6): 1850 - 1855.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
S. J. Getting, C. W. Lam, A. S. Chen, P. Grieco, and M. Perretti
Melanocortin 3 receptors control crystal-induced inflammation
FASEB J, November 1, 2006; 20(13): 2234 - 2241.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
L. Kang, K. W. McIntyre, K. M. Gillooly, Y. Yang, J. Haycock, S. Roberts, A. Khanna, T. F. Herpin, G. Yu, X. Wu, et al.
A selective small molecule agonist of the melanocortin-1 receptor inhibits lipopolysaccharide-induced cytokine accumulation and leukocyte infiltration in mice
J. Leukoc. Biol., October 1, 2006; 80(4): 897 - 904.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
N. R. Vulliemoz, E. Xiao, L. Xia-Zhang, M. Ferin, and S. L. Wardlaw
Melanocortin Modulation of Inflammatory Cytokine and Neuroendocrine Responses to Endotoxin in the Monkey
Endocrinology, April 1, 2006; 147(4): 1878 - 1883.
[Abstract] [Full Text] [PDF]

Home page
 Rheumatology (Oxford)Home page
N. Dalbeth and D. O. Haskard
Mechanisms of inflammation in gout
Rheumatology, September 1, 2005; 44(9): 1090 - 1096.
[Full Text] [PDF]

Home page
 J. Immunol.Home page
C. W. Lam, S. J. Getting, and M. Perretti
In Vitro and In Vivo Induction of Heme Oxygenase 1 in Mouse Macrophages following Melanocortin Receptor Activation
J. Immunol., February 15, 2005; 174(4): 2297 - 2304.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
S. J. Getting, C. Di Filippo, C. W. Lam, F. Rossi, and M. D'Amico
Investigation into the Potential Anti-Inflammatory Effects of Endothelin Antagonists in a Murine Model of Experimental Monosodium Urate Peritonitis
J. Pharmacol. Exp. Ther., July 1, 2004; 310(1): 90 - 97.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
A. Catania, S. Gatti, G. Colombo, and J. M. Lipton
Targeting Melanocortin Receptors as a Novel Strategy to Control Inflammation
Pharmacol. Rev., March 1, 2004; 56(1): 1 - 29.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
S. J. Getting, H. B. Schioth, and M. Perretti
Dissection of the Anti-Inflammatory Effect of the Core and C-Terminal (KPV) {alpha}-Melanocyte-Stimulating Hormone Peptides
J. Pharmacol. Exp. Ther., August 1, 2003; 306(2): 631 - 637.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. J. Getting, H. C. Christian, C. W. Lam, F. N. E. Gavins, R. J. Flower, H. B. Schioth, and M. Perretti
Redundancy of a Functional Melanocortin 1 Receptor in the Anti-inflammatory Actions of Melanocortin Peptides: Studies in the Recessive Yellow (e/e) Mouse Suggest an Important Role for Melanocortin 3 Receptor
J. Immunol., March 15, 2003; 170(6): 3323 - 3330.
[Abstract] [Full Text] [PDF]