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(Journal of Leukocyte Biology. 2001;69:69-74.)
© 2001 by Society for Leukocyte Biology

Adjuvant effect of -inulin is mediated by C3 fragments deposited on antigen-presenting cells

Krisztina Kerekes^*, Peter D. Cooper, József Prechl^*,, Mihály Józsi^*, Zsuzsa Bajtay^*, and Anna Erdei^*,

Research Group of the Hungarian Academy of Sciences
^* Department of Immunology, Eötvös Loránd University, Göd, Hungary
Division of Immunology and Cell Biology, John Curtin School of Medical Research, The Australian National University, Canberra, Australia

Correspondence: Anna Erdei, Department of Immunology, Eötvös Loránd University, H-2131 Göd, Jávorka S.u.14., Hungary.

The adjuvant effect of -inulin, a strong activator of the alternative complement pathway, is well-known, but its exact mechanism is not revealed yet. Here, we show that macrophages, isolated from the peritoneal cavity of -inulin-injected mice and used as antigen-presenting cells, enhance the proliferation of antigen-specific T-cells up to 2.5-fold when compared with macrophages of nontreated animals. This effect is abrogated by the presence of anti-C3 F(ab')₂ fragments and by prior decomplementation of the donor animals with CVF. It is demonstrated that treatment of mice with the adjuvant results in deposition of C3-fragments onto the surface of peritoneal macrophages, as does in vitro incubation of the cells with -inulin in the presence of fresh autologous serum. Prior incubation of macrophages with -inulin plus serum in vitro enhances subsequent C3 production. Because it has been shown earlier that CR1/2 expressed on activated T-cells and interacting with covalently bound C3-fragments plays an important role in the augmentation of the adaptive response, our present results reveal a mechanism that contributes to the adjuvant effect of -inulin and point to a further link between innate and adaptive immunity.

Key Words: adjuvanticity • complement activation • C3 deposition • T-cell response

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