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(Journal of Leukocyte Biology. 2000;68:748-756.)
© 2000 by Society for Leukocyte Biology

Activating Ly-49D NK receptors: expression and function in relation to ontogeny and Ly-49 inhibitor receptors

John R. Ortaldo*, Robin Winkler-Pickett* and Gordon Wiegand{dagger}

* Laboratory of Experimental Immunology, DBS, National Cancer Institute-FCRDC, and
{dagger} SAIC-Frederick, NCI-FCRDC, Frederick, Maryland

Correspondence: Dr. John R. Ortaldo, NCI-FCRDC, Bldg. 560, Rm. 31-93, Frederick, MD 21702-1201. E-mail: Ortaldo{at}mail.ncifcrf.gov

Developmental changes in the repertoire of activating Ly-49 family members have not been examined previously. In the present study, we have examined the expression and function of the activating Ly-49s (D and H) from birth through 8 weeks of age. We demonstrate that 1) activating Ly-49s are expressed early, 2) their expression intensity is not different from adult NK cells, and 3) activating receptors are functional. Examination of the inhibitory Ly-49s also demonstrated functional capacity immediately upon expression. To examine the kinetics of expression of the repertoire of activating Ly-49 members, we utilized five- and six-color flow cytometric analyses of NK cells from birth through adulthood. Previous studies examining the inhibitory Ly-49 repertoire have proposed that expression is regulated by the product rule. Our results indicated that Ly-49D, which recognizes H-2Dd, had a discordantly high coexpression of the inhibitory Ly-49s that recognized H-2Dd (Ly-49A and Ly-49G2). The product rule of Ly-49 expression does not explain the coexpression of selected activating and inhibitory receptors. This high level of coexpression of H-2Dd recognizing activating and inhibitory Ly- 49s suggests an in vivo selection or regulated coexpression.

Key Words: Ly-49 • NK • ontogeny • product rule




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