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(Journal of Leukocyte Biology. 2000;68:650-654.)
© 2000 by Society for Leukocyte Biology

Clustering on the forward surfaces of migrating neutrophils of a novel GPI-anchored protein that may regulate neutrophil adherence and migration

Yukiko Nakamura-Sato^*, Katsunori Sasaki, Hiroshi Watanabe, Yoshihiko Araki^* and Fujiro Sendo^*

^* Department of Immunology and Parasitology, Yamagata University, School of Medicine;
Department of Anatomy, Shinshu University, School of Medicine, Matsumoto; and
Department of Nursing, Yamagata University, School of Medicine, Yamagata, Japan

Correspondence: Fujiro Sendo, Department of Immunology and Parasitology, Yamagata University, School of Medicine, 2-2-2, Iida-Nishi, Yamagata 990-9585, Japan. E-mail: fsendo{at}med.id.yamagata-u.ac.jp

We previously reported a novel glycosylphosphatidylinositol (GPI)-anchored glycoprotein (tentatively designated GPI-80) on human leukocytes that may be involved in the regulation of neutrophil adherence and migration. In this study, we examined by immuno- and scanning electron microscopy, the distribution of GPI-80 on neutrophil surfaces. GPI-80 was diffusely distributed on the surface of resting neutrophils and on the peripheral areas of adherent cells after stimulation with N-formyl-methionyl-leucyl-phenylalanine. After longer stimulation (60 min), GPI-80 decreased in number and was again diffusely distributed on the surfaces of round neutrophils. Few GPI-80 were detected on the ventral surfaces of adherent neutrophils. Clusters of GPI-80 were detected on the forward surfaces of neutrophils transmigrating through pores of nitrocellulose membranes. These results may give a morphological background of possible role of GPI-80 for neutrophil extravasation.

Key Words: integrin • electron microscopy • localization

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