Laboratory of Experimental Immunology, Division of Basic Sciences, National Cancer Institute-FCRDC, Frederick, Maryland
Correspondence: Dr. L. Mason, Laboratory of Experimental Immunology, DBS, NCI-FCRDC, Bldg. 560, Rm. 31-93, Frederick, MD 21702-1201. E-mail: masonl{at}mail.ncifcrf.gov
Upon ligand recognition, members of the murine Ly-49 receptor family
can transmit inhibitory or activating signals that regulate NK cell
function. Ly-49A, G, and D have been shown to recognize the murine
class I molecule H-2Dd as a potential ligand. Recent
studies also have demonstrated also that Ly-49D+ NK cells
can lyse CHO cells, although the ligand responsible for this
recognition was not identified. Because allorecognition by NK cells may
be important in bone-marrow transplantation and because of the
overlapping class I recognition by these receptors, recognition of CHO
cells by Ly-49G and A was investigated. Our data suggest that Ly-49G
and probably A transmit inhibitory signals in response to CHO cells.
Receptor inhibition was assessed by examining NK lytic function,
IFN-
secretion, and DAP12 phosphorylation in response to CHO cells
by sorted subsets of Ly-49D vs. G B6 NK cells. Our results suggest that
CHO cells may express a common ligand(s) that is capable of engaging
Ly-49D, G, and possibly A in C576BL/6 NK cells. In addition to our
findings that Ly-49 inhibitory receptors also recognize CHO cells,
activating receptors other than Ly-49D are present in B6 mice that can
lyse CHO cells.
Key Words: Ly-49 • NK • CHO • IFN- • DAP-12
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