This Article Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Werr, J. Articles by Lindbom, L. Search for Related Content PubMed PubMed Citation Articles by Werr, J. Articles by Lindbom, L.

(Journal of Leukocyte Biology. 2000;68:553-560.)
© 2000 by Society for Leukocyte Biology

Engagement of ß₂ integrins induces surface expression of ß₁ integrin receptors in human neutrophils

Department of Physiology and Pharmacology, Karolinska Institutet, S-171 77 Stockholm, Sweden

Correspondence: Joachim Werr, Department of Physiology and Pharmacology, Karolinska Institutet, S-171 77 Stockholm, Sweden. E-mail: joachim.werr{at}fyfa.ki.se

Induction of ß₁ integrin (CD49/CD29) expression in polymorphonuclear leukocytes (PMN) has been shown to be associated with transendothelial migration recently. Yet, ß₁ integrin expression is relatively insensitive to cell activation with soluble agonists, such as N-formyl-methionyl-leucyl-phenylalanine (fMLP). We hypothesized that ß₂ integrins (CD11/CD18), critically involved in PMN adhesion and extravasation, may play a role in regulating ß₁ integrin expression in PMN. Antibody cross-linking of CD18, mimicking adhesion-dependent engagement of ß₂ integrins, resulted in rapid, tyrosine kinase-dependent upregulation of ß₁ integrins. This response was potentiated by simultaneous chemoattractant (fMLP) stimulation of PMN. Moreover, upregulation of ß₁ integrins evoked by CD18 cross-linking was found to support adhesion of fMLP-stimulated PMN to matrix proteins and also was critical for the ability of PMN to migrate in collagen gels in response to a gradient of fMLP. Taken together, these data demonstrate that engagement of ß₂ integrins in human PMN induces ß₁ integrin expression in these cells of significance for their migration in the extravascular tissue. Thus, ß₂ integrins may serve the function to regulate PMN locomotion in extravascular tissue via receptor crosstalk with ß₁ integrins.

Key Words: inflammation • leukocytes • adhesion • extravasation • migration

This article has been cited by other articles:

				S. Lee, K. Bowrin, A. R. Hamad, and S. Chakravarti Extracellular Matrix Lumican Deposited on the Surface of Neutrophils Promotes Migration by Binding to {beta}2 Integrin J. Biol. Chem., August 28, 2009; 284(35): 23662 - 23669. [Abstract] [Full Text] [PDF]

				D G Downey, S C Bell, and J S Elborn Neutrophils in cystic fibrosis Thorax, January 1, 2009; 64(1): 81 - 88. [Abstract] [Full Text] [PDF]

				B. Petri, M. Phillipson, and P. Kubes The Physiology of Leukocyte Recruitment: An In Vivo Perspective J. Immunol., May 15, 2008; 180(10): 6439 - 6446. [Abstract] [Full Text] [PDF]

				L. M. Lavigne, X. M. O'Brien, M. Kim, J. W. Janowski, J. E. Albina, and J. S. Reichner Integrin Engagement Mediates the Human Polymorphonuclear Leukocyte Response to a Fungal Pathogen-Associated Molecular Pattern J. Immunol., June 1, 2007; 178(11): 7276 - 7282. [Abstract] [Full Text] [PDF]

				C. E. Green, U. Y. Schaff, M. R. Sarantos, A. F. H. Lum, D. E. Staunton, and S. I. Simon Dynamic shifts in LFA-1 affinity regulate neutrophil rolling, arrest, and transmigration on inflamed endothelium Blood, March 1, 2006; 107(5): 2101 - 2111. [Abstract] [Full Text] [PDF]

				A. Ortiz-Stern and C. Rosales Fc{gamma}RIIIB stimulation promotes {beta}1 integrin activation in human neutrophils J. Leukoc. Biol., May 1, 2005; 77(5): 787 - 799. [Abstract] [Full Text] [PDF]

				A. R. Burns, C. W. Smith, and D. C. Walker Unique Structural Features That Influence Neutrophil Emigration Into the Lung Physiol Rev, April 1, 2003; 83(2): 309 - 336. [Abstract] [Full Text] [PDF]

				J. Dangerfield, K. Y. Larbi, M.-T. Huang, A. Dewar, and S. Nourshargh PECAM-1 (CD31) Homophilic Interaction Up-Regulates {alpha}6{beta}1 on Transmigrated Neutrophils In Vivo and Plays a Functional Role in the Ability of {alpha}6 Integrins to Mediate Leukocyte Migration through the Perivascular Basement Membrane J. Exp. Med., November 4, 2002; 196(9): 1201 - 1212. [Abstract] [Full Text] [PDF]

				J. D. Borgquist, M. T. Quinn, and S. D. Swain Adhesion to extracellular matrix proteins modulates bovine neutrophil responses to inflammatory mediators J. Leukoc. Biol., May 1, 2002; 71(5): 764 - 774. [Abstract] [Full Text] [PDF]

				A. K. McNally and J. M. Anderson {beta}1 and {beta}2 Integrins Mediate Adhesion during Macrophage Fusion and Multinucleated Foreign Body Giant Cell Formation Am. J. Pathol., February 1, 2002; 160(2): 621 - 630. [Abstract] [Full Text] [PDF]

				Y.-C. Shen, C.-J. Chou, W.-F. Chiou, and C.-F. Chen Anti-Inflammatory Effects of the Partially Purified Extract of Radix Stephaniae tetrandrae: Comparative Studies of Its Active Principles Tetrandrine and Fangchinoline on Human Polymorphonuclear Leukocyte Functions Mol. Pharmacol., November 1, 2001; 60(5): 1083 - 1090. [Abstract] [Full Text]