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* Intramural Research Support Program, SAIC Frederick and Laboratory of Molecular Immunoregulation, Division of Basic Sciences, NCI-FCRDC, Frederick, Maryland;
Institute of Toxicology, ETH, Schwerzenbach, Switzerland;
Laboratory of Molecular Immunology, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, and Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia; and
Ludwig Institute for Cancer Research, New York Branch at Sloan-Kettering Memorial Cancer Center
Correspondence: Alexander N. Shakhov, IRSP, SAIC Frederick, Bldg. 560, Rm. 31-33, NCI-FCRDC, Frederick, MD 21702. E-mail: shakhova{at}mail.ncifcrf.gov
Mice with combined lymphotoxin-
(LT) and tumor necrosis
factor (TNF) deficiencies show defects in the structure of peripheral
lymphoid organs such as spleen, lymph nodes, and gut-associated
lymphoid tissues. To identify genes associated with this defective
phenotype in spleen, we applied a gene profiling approach, including
subtractive cloning and gene array hybridizations, to mice with
combined TNF/LT deficiency. The differentially expressed genes
identified by these techniques was then evaluated by Northern blot
analysis for splenic expression in knockout mice with single LT or
single TNF deficiency. Most of the genes detected in this analysis are
directly or indirectly associated with disrupted LT and not TNF
signaling.
Key Words: gene arrays • subtractive cloning • knockout mice • spleen
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