Unité INSERM U167, Institut Pasteur, Lille, France
Correspondence: Dr. Monique Capron, Unité INSERM U167, Institut Pasteur de Lille, 1 rue du Prof. Calmette, BP 245, 59019 Lille Cedex, France. E-mail: monique.capron{at}pasteur-lille.fr
Polymorphonuclear neutrophils (PMN) are phagocytic cells, able to
secrete a large range of cytokines, including inflammatory cytokines,
chemokines, as well as the Th1 cytokines interferon-
(IFN-) and
interleukin (IL)-12. Although PMN do not seem to express IL-10 and
IL-13, no information exists on the ability of PMN to produce IL-4.
Therefore intracellular flow cytometry was performed in the presence or
absence of Brefeldin A. Similarly to eosinophils, freshly isolated
neutrophils from normal donors contained low amounts of IL-4, which
significantly increased upon culture with Brefeldin A
(P < 0001). Immunostaining performed on cytospin
preparations of normal granulocytes confirmed the presence of
intracellular IL-4. Using a highly sensitive ELISA, the levels of IL-4
secreted by cultured PMN and peripheral blood mononuclear cells (PBMC)
were compared. PBMC secrete up to 60 times more IL-4 as PMN but, in the
presence of calcium ionophore, only PMN showed a slight but significant
increase in IL-4 secretion (P < 0.05). In conclusion,
we report here the presence within human PMN of intracellular IL-4,
which can at least partly be released under calcium ionophore
stimulation. The relevance of this production of IL-4 by human PMN is
discussed.
Key Words: eosinophils • intracellular flow cytometry • enzyme-linked immunosorbent assay
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