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Journal of Leukocyte Biology, Vol 67, Issue 6 780-784, Copyright © 2000 by Society for Leukocyte Biology

JOURNAL ARTICLE

Transient infiltration of neutrophils into the thymus in association with apoptosis induced by whole-body X-irradiation

E Uchimura, N Watanabe, O Niwa, M Muto and Y Kobayashi
Department of Biomolecular Science, Faculty of Science, Toho University, Funabashi, Japan.

Generally, the process of apoptosis does not cause leakage of noxious cytosolic contents and is therefore non-inflammatory. However, as previously shown, macrophages ingesting apoptotic CTLL-2 cells produced pro-inflammatory cytokines, particularly interleukin-8 (IL-8) and macrophage inflammatory protein-2 (MIP-2), a murine IL-8 homolog. This predicted that rapid and massive apoptosis may induce neutrophil accumulation in vivo. In this study, we tested this prediction by inducing apoptosis by whole-body X-irradiation in mice. After exposure to 4 Gy X-ray irradiation, mice exhibited considerable apoptosis of thymic cells, which was associated with transient infiltration of neutrophils as well as MIP-2 mRNA expression. In contrast, in p53-deficient mice in which irradiation-induced apoptosis was suppressed, as has been reported, infiltration of neutrophils into the thymus was less than that found in p53+/+ mice. Taken together, these results suggest that massive and rapid apoptosis can result in infiltration of neutrophils.

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