This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Uchimura, E. Articles by Kobayashi, Y. Search for Related Content PubMed PubMed Citation Articles by Uchimura, E. Articles by Kobayashi, Y.

Journal of Leukocyte Biology, Vol 67, Issue 6 780-784, Copyright © 2000 by Society for Leukocyte Biology

JOURNAL ARTICLE

Transient infiltration of neutrophils into the thymus in association with apoptosis induced by whole-body X-irradiation

E Uchimura, N Watanabe, O Niwa, M Muto and Y Kobayashi
Department of Biomolecular Science, Faculty of Science, Toho University, Funabashi, Japan.

Generally, the process of apoptosis does not cause leakage of noxious cytosolic contents and is therefore non-inflammatory. However, as previously shown, macrophages ingesting apoptotic CTLL-2 cells produced pro-inflammatory cytokines, particularly interleukin-8 (IL-8) and macrophage inflammatory protein-2 (MIP-2), a murine IL-8 homolog. This predicted that rapid and massive apoptosis may induce neutrophil accumulation in vivo. In this study, we tested this prediction by inducing apoptosis by whole-body X-irradiation in mice. After exposure to 4 Gy X-ray irradiation, mice exhibited considerable apoptosis of thymic cells, which was associated with transient infiltration of neutrophils as well as MIP-2 mRNA expression. In contrast, in p53-deficient mice in which irradiation-induced apoptosis was suppressed, as has been reported, infiltration of neutrophils into the thymus was less than that found in p53+/+ mice. Taken together, these results suggest that massive and rapid apoptosis can result in infiltration of neutrophils.

This article has been cited by other articles:

				K. C. Flanders, B. M. Ho, P. R. Arany, C. Stuelten, M. Mamura, M. O. Paterniti, A. Sowers, J. B. Mitchell, and A. B. Roberts Absence of Smad3 Induces Neutrophil Migration after Cutaneous Irradiation: Possible Contribution to Subsequent Radioprotection Am. J. Pathol., July 1, 2008; 173(1): 68 - 76. [Abstract] [Full Text] [PDF]

				T. Shibata, K. Nagata, and Y. Kobayashi Cutting Edge: A Critical Role of Nitrogen Oxide in Preventing Inflammation upon Apoptotic Cell Clearance J. Immunol., September 15, 2007; 179(6): 3407 - 3411. [Abstract] [Full Text] [PDF]

				T. Iyoda, K. Nagata, M. Akashi, and Y. Kobayashi Neutrophils Accelerate Macrophage-Mediated Digestion of Apoptotic Cells In Vivo as Well as In Vitro J. Immunol., September 15, 2005; 175(6): 3475 - 3483. [Abstract] [Full Text] [PDF]

				A. Devitt, K. G. Parker, C. A. Ogden, C. Oldreive, M. F. Clay, L. A. Melville, C. O. Bellamy, A. Lacy-Hulbert, S. C. Gangloff, S. M. Goyert, et al. Persistence of apoptotic cells without autoimmune disease or inflammation in CD14-/- mice J. Cell Biol., December 20, 2004; 167(6): 1161 - 1170. [Abstract] [Full Text] [PDF]

				C. Berruyer, F. M. Martin, R. Castellano, A. Macone, F. Malergue, S. Garrido-Urbani, V. Millet, J. Imbert, S. Dupre, G. Pitari, et al. Vanin-1-/- Mice Exhibit a Glutathione-Mediated Tissue Resistance to Oxidative Stress Mol. Cell. Biol., August 15, 2004; 24(16): 7214 - 7224. [Abstract] [Full Text] [PDF]

				S. I. Hodgetts and M. D. Grounds Irradiation of dystrophic host tissue prior to myoblast transfer therapy enhances initial (but not long-term) survival of donor myoblasts J. Cell Sci., October 15, 2003; 116(20): 4131 - 4146. [Abstract] [Full Text] [PDF]

				M. Lucas, L. M. Stuart, J. Savill, and A. Lacy-Hulbert Apoptotic Cells and Innate Immune Stimuli Combine to Regulate Macrophage Cytokine Secretion J. Immunol., September 1, 2003; 171(5): 2610 - 2615. [Abstract] [Full Text] [PDF]

				K. Kurosaka, N. Watanabe, and Y. Kobayashi Potentiation by human serum of anti-inflammatory cytokine production by human macrophages in response to apoptotic cells J. Leukoc. Biol., June 1, 2002; 71(6): 950 - 956. [Abstract] [Full Text] [PDF]

				T. Waku, T. Fujiwara, J. Shao, T. Itoshima, T. Murakami, M. Kataoka, S. Gomi, J. A. Roth, and N. Tanaka Contribution of CD95 Ligand-Induced Neutrophil Infiltration to the Bystander Effect in p53 Gene Therapy for Human Cancer J. Immunol., November 15, 2000; 165(10): 5884 - 5890. [Abstract] [Full Text] [PDF]

				S. Faouzi, B. E. Burckhardt, J. C. Hanson, C. B. Campe, L. W. Schrum, R. A. Rippe, and J. J. Maher Anti-Fas Induces Hepatic Chemokines and Promotes Inflammation by an NF-kappa B-independent, Caspase-3-dependent Pathway J. Biol. Chem., December 21, 2001; 276(52): 49077 - 49082. [Abstract] [Full Text] [PDF]