Accuri C6 Flow Cytometer System

This Article
Right arrow Full Text (PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sylwester, A.
Right arrow Articles by Soll, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sylwester, A.
Right arrow Articles by Soll, D. R.

Journal of Leukocyte Biology, Vol 63, Issue 2 233-244, Copyright © 1998 by Society for Leukocyte Biology

JOURNAL ARTICLE

The invasive and destructive behavior of HIV-induced T cell syncytia on collagen and endothelium

A Sylwester, K Daniels and DR Soll
Department of Biological Sciences, The University of Iowa, Iowa City 52442, USA.

HIV-induced syncytia of the CD4+ SUP-T1 T cell line mimic the subcellular organization of single cells and are able to crawl like single cells through extension of giant pseudopods. Because syncytia have been demonstrated in lymphoid tissue of HIV-positive individuals, their behavior has been investigated on more natural substrata, including dehydrated collagen, hydrated collagen, endothelial monolayers, and endothelial monolayers grown on collagen cushions. On hydrated collagen gels, both individual SUPT1 cells and syncytia form unusually long cylindrical projections that possess pseudopodial ends and are highly dynamic. Syncytia penetrate collagen gels through extension of these projections and disrupt their integrity. When incubated on endothelium, both single cells and syncytia readily traverse the monolayer through holes, and when incubated on endothelium supported by a collagen cushion, syncytia generate large holes through the monolayer, penetrate the monolayer, and disrupt the collagen gel through extension of long, complex projections. Invading syncytia also release viruses in a polarized fashion which adhere to and are taken up in vesicles by the endothelium. It is suggested that the destructive behaviors of syncytia which have been demonstrated in vitro may have correlates in vivo.


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
D. Shutt and D. Soll
HIV-induced T-cell syncytia release a two component T-helper cell chemoattractant composed of Nef and Tat
J. Cell Sci., January 11, 1999; 112(22): 3931 - 3941.
[Abstract] [PDF]