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Journal of Leukocyte Biology, Vol 62, Issue 6 911-915, Copyright © 1997 by Society for Leukocyte Biology

JOURNAL ARTICLE

Monocyte chemoattractant protein-2 is a potent agonist of CCR2B

UM Moore, JM Kaplow, RD Pleass, SW Castro, K Naik, CN Lynch, S Daly, AG Roach, M Jaye and RJ Williams
Rhone-Poulenc Rorer Ltd., Dagenham Research Centre, Essex, United Kingdom. Una.Moore@DRC.RP-Rorer.rp.fr

The binding and functional activity of the CC chemokines monocyte chemoattractant protein-1 (MCP-1), MCP-2, and MCP-3 have been characterized using Chinese hamster ovary DXB-11 cells transfected with the chemokine receptor CCR2B. Receptor binding studies demonstrated that 125I-labeled MCP-1 bound to a single class of high-affinity receptors with a Kd of 0.14 (0.07-0.32) nM. In competition studies MCP-1, MCP-2, and MCP-3 completely inhibited 125I-labeled MCP-1 binding with Ki values of 0.3 (0.16-0.46), 8.8 (3.4-26), and 12.2 (0.6-22) nM, respectively. In calcium mobilization studies, MCP-1 and MCP-3 induced robust elevations in intracellular calcium concentrations, whereas MCP-2 was only weakly active. In contrast, using changes in extracellular acidification rate as a functional readout, all three chemokines were identified as potent agonists of CCR2B. These data demonstrate that MCP-2, in addition to MCP-1 and MCP-3, is a potent agonist of CCR2B and furthermore that MCP-2 activates either different or a subset of the signaling pathways activated by MCP-1 and MCP-3.


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